Humanization and Characterization of an Anti-Human TNF-α Murine Monoclonal Antibody
Figure 2
Amino acid sequence alignment of the VH (A) and VL (B) domains of m357 and PPS4.
The sequences of PPS4 Fv which were the most homologous to m357 Fv used as the acceptor of human surface residues for m357 humanization is shown as PPS4VL and PPS4VH for comparison. CDR residues were within brackets. Conserved surface residues were marked with clear boxes, and non-conserved surface residues were marked with shadowed boxes. Amino acid sequences are numbered according to the Kabat's convention[33].