Environmental Exposure of the Mouse Germ Line: DNA Adducts in Spermatozoa and Formation of De Novo Mutations during Spermatogenesis
Figure 3
Mutation frequencies in cauda spermatozoa from B[a]P-exposed male germ cells at different stages of spermatogenesis.
The male germ cell stage is indicated along with day of sacrifice. Cauda spermatozoa = CASZ (untreated, n = 3); epididymal spermatozoa = ESZ (n = 4); spermatids = ST (n = 6); spermatocytes = SC (n = 5); differentiating spermatogonia = Diff. SG (n = 6); stem cell spermatogonia = Stem cell SG (n = 6). Mf values are given as means (per 106 pfu) ±SE. Differences in mean Mf were tested statistically (Mann-Whitney); The B[a]P-exposed were compared with untreated control, and B[a]P-exposed at 119 days were also compared with its time-matched corn oil treated control. Statistically significant differences are indicated (p = 0.029).