COE Loss-of-Function Analysis Reveals a Genetic Program Underlying Maintenance and Regeneration of the Nervous System in Planarians
Figure 6
CNS regeneration defects following knockdown of COE-regulated genes.
(A–D) Animals were fed control, scna-2, nkx2l and pou4l-1 bacterially-expressed dsRNA (indicated to the left of each panel), amputated pre-pharyngeally and allowed to regenerate. Ten-day regenerates were imaged live (A–D), killed and immunostained with anti-SYNAPSIN or processed for fluorescent in situ hybridization to ChAT or npl (N≥4). (E–F) Brain size estimated by measuring head area stained by anti-SYNAPSIN or in situ hybridization to ChAT and normalized by the length of animal for control, scna-2, nkx2l, and pou4l-1 RNAi planarians. (G) Quantification of npl+ cells normalized by brain size measured from ChAT stain in F (N≥4 animals in each group); the total number of npl+ cells counted is indicated within each bar. Error bars in all graphs are s.d. from the mean; *P<0.05, Student's t-test. Anterior is up in A–D. Scale bars = 100 µm.