Abstract
Species of the genus Chuquiraga Juss., Asteraceae, Barnadesioideae, are widely marketed in South America as medicinal plants, despite their relatively uncharacterized phytochemistry. The present study aimed to profile phenolic compounds in commercial samples of three Chuquiraga species (C. jussieui J.F.Gmel., C. spinosa Less., and C. weberbaueri Tovar) from Ecuador and Peru by a high-performance liquid chromatography with diode array detection fingerprinting method combined with multivariate statistical analyses for species classification. From the obtained HPLC fingerprint chromatograms, the traces at 270 nm were selected and submitted to time shifts of peak corrections using the parametric time warping algorithm. Hierarchical clustering analysis, principal components analysis, orthogonal partial least squares discriminant analysis, and k-nearest neighbor measurements were used to evaluate similarities and establish classification models for species discrimination. Based on these analyses, a high percentage of correct classifications (between 83 and 100%) allowed the prediction of the taxonomic identity of unlabeled commercial samples and its geographical origin. Acetophenones (p-hydroxyacetophenone, p-hydroxyacetophenone 4-O-glucoside, p-hydroxyacetophenone 4-O-(6-O-apiosyl)-glucoside), phenylpropanoids (3-O-caffeoylquinic acid, 5-O-caffeoylquinic acid), and flavonoids (quercetin 3-O-rutinoside, quercetin 3-O-glucoronide, kaempferol 3-O-rutinoside) were identified as main constituents of the investigated species.
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17 November 2021
A Correction to this paper has been published: https://doi.org/10.1007/s43450-021-00209-7
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Acknowledgements
The authors express their gratitude to Dr. Ricardo R. da Silva (School of Pharmaceutical Sciences of Ribeirão Preto, USP) for its support in the implementation of PTW script.
Funding
Financial support was provided by the São Paulo Research Foundation (FAPESP) (grant no. 2014/16850–6 and 2014/26866–7), the Coordination for the Improvement of Higher Education Personnel (CAPES: code 001), and the National Council for Scientific and Technological Development (CNPq) for fellowship and funding.
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GVC carried out the experiments and wrote the manuscript; GFP and RC guided the multivariate analyses and revised and corrected the manuscript; MDC helped with the isolation procedures and chromatographic fingerprint data acquisition; PLF, MM, JS, and MG conducted the identification of Chuquiraga species; FBC coordinated the project and was responsible for its final written version. All authors read and approved the final manuscript submission.
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This article is part of a Special Issue to celebrate the 35th anniversary of the Brazilian Journal of Pharmacognosy
This article has been corrected to add an article note and update the size of the chemical structures which are meant to be placed in two columns. This will allow the readers to observe the stereochemistry of the structures. This will allow the readers to observe the stereochemistry of the structures.
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Ccana-Ccapatinta, G.V., Padilla-Gonzalez, G.F., Ferreira, P.L. et al. Phenolic Profiling of Medicinal Species of Chuquiraga, Asteraceae, by HPLC Fingerprinting. Rev. Bras. Farmacogn. 31, 689–697 (2021). https://doi.org/10.1007/s43450-021-00177-y
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DOI: https://doi.org/10.1007/s43450-021-00177-y