Skip to main content
SpringerLink
Log in

Pseudo-nitzschia fukuyoi (Bacillariophyceae), a domoic acid-producing species from Nha Phu Bay, Khanh Hoa Province, Vietnam

  • Original Article
  • Chemistry and Biochemistry
  • Published:
Fisheries Science Aims and scope Submit manuscript

Abstract

Two strains of Pseudo-nitzschia fukuyoi isolated from Vietnamese waters produce domoic acid, a toxin responsible for amnesic shellfish poisoning. Species identification was based on detailed morphological observation using a transmission electron microscope and also molecular data on large subunit (LSU) and the second internal transcribed spacer (ITS2) with NCBI nucleotide Blast (blastn). Toxin productivity of the two strains was confirmed and their range were 3.85–4.54 pg/cell by analyses using LC–MS/MS. This is the first report of occurrence of P. fukuyoi in Vietnamese waters, and the first confirmation of productivity of domoic acid of the species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Lim HC, Su SNP, Mohamed-Ali H, Kotaki Y, Leaw CP, Lim PT (2010) Toxicity of diatom Pseudo-nitzschia (Bacillariophyceae) analyzed using high performance liquid chromatography (HPLC). J Sci Technol Trop 6:S116–S119

  2. Lim HC, Leaw CP, Su SNP, Teng ST, Usup G, Noor NM, Lundholm N, Kotaki Y, Lim PT (2012) Morphology and molecular characterization of Pseudo-nitzschia (Bacillariophyceae) from Malaysian Borneo, including the new species Pseudo-nitzschiacircumpora sp. nov. J Phycol 48(5):1232–1247

    Article  CAS  Google Scholar 

  3. Lim HC, Teng ST, Leaw CP, Lim PT (2013) Three novel species in the Pseudo-nitzschia pseudo delicatissima complex: P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia. J Phycol 49(5):902–916

    CAS  Google Scholar 

  4. Teng ST, Leaw CP, Lim HC, Lim PT (2013) The genus Pseudo-nitzschia (Bacillariophyceae) in Malaysia, including new records and a keyto species inferred from morphology-based phylogeny. Bot Mar 56:375–398

    Article  Google Scholar 

  5. Teng ST, Lim HC, Lim PT, Dao VH, Bates SS, Leaw CP (2014) Pseudo-nitzschiakodamae sp. nov. (Bacillariophyceae), a toxigenic species from the Strait of Malacca, Malaysia. Harmful Algae 34:17–28. doi:10.1016/j.hal.2014.02.005

    Article  Google Scholar 

  6. Skov J, Ton TP, Do TBL (2004) Bacillariophyceae. In: Potentially toxic microalgae of Vietnamese waters. In: Larsen J, Nguyen NL (eds) Opera Botanica, vol 140. Council for Nordic Publications in Botany, Copenhagen, pp 23–51

    Google Scholar 

  7. Doan NH, Tin NT, Anh NTM, Lam NN (2013) Species composition and cell density variation of diatoms Pseudo-nitzschia spp. in coastal waters of Khanh Hoa, Vietnam. In: Bui HL et al (eds) Proceedings of the international conference on “Bien Dong 2012”, 12–14 September, 2012 (in Vietnamese with English abstract), pp 159–268

  8. Dao VH, Lim PT, Ky PX, Takata Y, Teng ST, Omura T, Fukuyo Y, Kodama M (2014) Diatom Pseudo-nitzschia cf. caciantha (Bacillariophyceae), the most likely source of domoic acid contamination in the thorny oyster Spondylusversicolor Schreibers 1793 in Nha Phu Bay, Khanh Hoa Province, Vietnam. Asian Fish Sci 27:16–29

    Google Scholar 

  9. DaoVH, Takata Y, Omura T, Sato S, Fukuyo Y, Kodama M (2009) Seasonal variation of domoic acid in Spondylus versicolor in association with that in plankton samples in Nha Phu Bay, Khanh Hoa, Vietnam. Fish Sci 75:507–512

    Article  CAS  Google Scholar 

  10. Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds) Culture of marine invertebrate animals. Plenum Press, New York, pp 26–60

    Google Scholar 

  11. Bates SS, de Freltas ASW, Milley JE, Pocklington R, Quilliam MA, Smith JC, Worms J (1991) Controls on domoic acid production by the diatom Nitzschiapungens f. multiseries in culture nutrients and irradiance. Can J Fish Aquat Sci 48:1136–1144

    Article  CAS  Google Scholar 

  12. Lundholm N, Bates SS, Baugh KA, Bill BD, Connell LB, Léger C, Trainer VL (2012) Cryptic and pseudo-cryptic diversity in diatoms-with descriptions of Pseudo-nitzschiahasleana sp. nov. and P. fryxelliana sp. nov. J Phycol 48(2):436–454

    Article  Google Scholar 

  13. Bargu S, Koray T, Lundholm N (2002) First report of Pseudo-nitzschia calliantha Lundholm, Moestrup and Hasle 2003, a new potentially toxic species from Turkish Coast. J Fish Aqua Sci 19(3–4):479–483

    Google Scholar 

  14. White T, Bruns T, Lee S, Taylor J, Innis M, Gelfand D, Shinsky J, White T (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA et al (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322

    Chapter  Google Scholar 

  15. Scholin CA, Herzog M, Sogin M, Anderson DM (1994) Identification of group-and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragment of the LSU rRNA gene. J Phycol 30(6):999–1011

    Article  CAS  Google Scholar 

  16. Orive E, Pérez-Aicua L, David H, García-Etxebarria K, Laza-Martínez A, Seoane S, Miguel I (2013) The genus Pseudo-nitzschia (Bacillariophyceae) in a temperate estuary with description of two new species: Pseudo-nitzschia plurisecta sp. nov. and Pseudo-nitzschia abrensis sp. nov. J Phycol 49:1192–1206

    Article  CAS  Google Scholar 

  17. Lundholm N, Moestrup Ø, Hasle GR, Hoef-Emden K (2003) A study of the Pseudo-nitzschia pseudodelicatissima/cuspidata complex (Bacillariophyceae): what is P. pseudodelicatissima? J Phycol 39:797–813

    Article  Google Scholar 

  18. Amato A, Montresor M (2008) Morphology, phylogeny, and sexual cycle of Pseudo-nitzschia mannii sp. nov. (Bacillariophyceae): a pseudocryptic species within the P. pseudodelicatissima complex. Phycologia 47:487–497

    Article  Google Scholar 

  19. Lelong AL, Hégaret HH, Soudant P, Bates SS (2012) Pseudo-nitzschia (Bacillariophyceae) species, domoic acid and amnesic shellfish poisoning: revisiting previous paradigms. Phycologia 51:168–216

    Article  CAS  Google Scholar 

  20. Fernandes LF, Hubbard KA, Richlen M, Smith J, Bates SS, Ehrman J, Léger C, Mafra LL Jr, Kulis D, Quilliam M, Erdner D, Libera K, McCauley L, Anderson D (2014) Diversity and toxicity of the diatom Pseudo-nitzschia Peragallo in the Gulf of Maine, Northwestern Atlantic Ocean. Deep Sea Res II 103:139–162

    Article  CAS  Google Scholar 

  21. Trainer VL, Bates SS, Lundholm N, Thessen AE, Cochlan WP, Adams NG, Trick CG (2012) Pseudo-nitzschia physiological ecology, phylogeny, toxicity, monitoring and impacts on ecosystem health. Harmful Algae 14:271–300

    Article  Google Scholar 

Download references

Acknowledgments

This study was funded by project 106.99-2010.22—NAFOSTED, Vietnam. The authors wish to express the deep thank to Dr. Stephen S. Bates (Fisheries and Oceans Canada, Gulf Fisheries Centre, Canada) for the help to improve the manuscript, both in scientific ideas and English correction.

Author information

Authors and Affiliations

  1. Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da, Nhatrang, Vietnam

    Ha Viet Dao, Vy Bao Phan & Ky Xuan Pham

  2. Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, 94300, Sarawak, Malaysia

    Sing Tung Teng

  3. Tohoku National Fisheries Research Institute, 3-27-5 Shinhama, Shiogama, Miyagi, 985-0001, Japan

    Hajime Uchida & Toshiyuki Suzuki

  4. Bachok Marine Research Station, Institute of Ocean and Earth Sciences, University of Malaya, 16310 Bachok, Kelantan, Malaysia

    Chui Pin Leaw & Po Teen Lim

Authors
  1. Ha Viet Dao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vy Bao Phan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sing Tung Teng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hajime Uchida
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chui Pin Leaw
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Po Teen Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Toshiyuki Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ky Xuan Pham
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ha Viet Dao.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dao, H.V., Phan, V.B., Teng, S.T. et al. Pseudo-nitzschia fukuyoi (Bacillariophyceae), a domoic acid-producing species from Nha Phu Bay, Khanh Hoa Province, Vietnam. Fish Sci 81, 533–539 (2015). https://doi.org/10.1007/s12562-015-0864-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12562-015-0864-9

Keywords

Search

Navigation