Abstract
Nannochloropsis oculata CS179, a unicellular marine microalga, is rich in long-chain polyunsaturated fatty acids (LCPUFAs). Elongase and desaturase play a key role in the biosynthesis of PUFAs. A new elongase gene, which encodes 322 amino acids, was identified via RT-PCR and 5′ and 3′ RACE. The sequence of the elongase gene was blast-searched in the NCBI GenBank and showed a similarity to those of the cryptosporidium. But the NJ-tree revealed that the N. oculata CS179 elongase clustered with those of the microalgae Phaeodactylum tricornutum, Ostreococcus tauri and Thalassiosira pseudonana.
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Pan, K., Ma, X., Yu, J. et al. Cloning and phylogenetic analysis of a fatty acid elongase gene from Nannochloropsis oculata CS179. J. Ocean Univ. China 8, 392–398 (2009). https://doi.org/10.1007/s11802-009-0392-2
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DOI: https://doi.org/10.1007/s11802-009-0392-2