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Elovaara, Samu; Eronen-Rasimus, Eeva; Asmala, Eero; Tamelander, Tobias; Kaartokallio, Hermanni (2021): Monitoring of the growth of phytoplankton batch cultures in part 1 (dissolved organic matter release) of the microcosm experiment, Gulf of Finland, Baltic Sea [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.937712, In: Elovaara, S et al. (2021): Phytoplankton and bacterial production, 14C-transfer and optical characteristics of dissolved organic matter (DOM) from a microcosm experiment, Gulf of Finland, Baltic Sea [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.937723

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Abstract:
The data were collected from an experiment using phytoplankton cultures (Apocalathium malmogiense and Rhodomonas marina). The aim of the experiment was to study carbon cycling among phytoplankton and bacteria, and the effects on the dissolved organic matter (DOM) pool. The experiment was conducted at Tvärminne Zoological Station, Hanko, Finland with non-axenic unialgal phytoplankton cultures and bacteria originating from the Baltic Sea. The experiment was conducted between Dec. 2017 and Apr. 2018. The experiment consisted of two parts, the DOM release experiment (part 1) and the DOM consumption experiment (part 2). Separate triplicate batch cultures of both phytoplankton species were grown for each experiment. In the DOM release experiment the cultures were grown for over 4 months and three day-long incubations (key point incubations, KPI's) were initiated on three occasions; the first KPI at early exponential growth phase and the second and third KPI's when the phytoplankton had grown more abundant.
This data table contains measurements collected during monitoring of the growth of phytoplankton batch cultures in part 1 of the experiment (DOM release experiment), i.e., before and in between of the KPIs. These phytoplankton cultures were used in the three key point incubations of the DOM release experiment. The variables measured during the monitoring, and included in this data file, are phytoplankton abundance, abundance of A. malmogiense cells with lower chlorophyll a fluorescence, percentage of phytoplankton cells with intact membranes, and optical properties of DOM. The experimental design is explained in figure 1 of the associated publication.
Keyword(s):
14C; Bacteria; bacterial production; Baltic Sea; CDOM; DOM; FDOM; Laboratory experiment; Laboratory strains; Phytoplankton; primary production
Supplement to:
Elovaara, Samu; Eronen-Rasimus, Eeva; Asmala, Eero; Tamelander, Tobias; Kaartokallio, Hermanni (2021): Contrasting patterns of carbon cycling and dissolved organic matter processing in two phytoplankton–bacteria communities. Biogeosciences, 18(24), 6589-6616, https://doi.org/10.5194/bg-18-6589-2021
Coverage:
Latitude: 59.856521 * Longitude: 23.258936
Date/Time Start: 2017-10-31T00:00:00 * Date/Time End: 2018-05-02T00:00:00
Event(s):
Amalmogiense_SHTV-2_FINMARI * Latitude: 59.856521 * Longitude: 23.258936 * Date/Time: 2002-01-01T00:00:00 * Location: Tvärminne, Storfjärden, Finland * Method/Device: LIMNOS water sampler (LIMNOSWS) * Comment: Collection site of Apocalathium malmogiense culture SHTV-2 of FINMARI culture collection/SYKE Marine Research Centre. Coordinates are approximates as exact sampling location is not recorded.
Rmarina_Crypto08-A2_FINMARI * Latitude: 59.856521 * Longitude: 23.258936 * Date/Time: 2008-01-01T00:00:00 * Location: Tvärminne, Storfjärden, Finland * Method/Device: LIMNOS water sampler (LIMNOSWS) * Comment: Collection site of Rhodomonas marina culture Crypto08-A2 of FINMARI culture collection/SYKE Marine Research Centre. Coordinates are approximates as exact sampling location is not recorded.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventElovaara, Samu
2Type of studyStudy typeElovaara, Samu
3Temperature, waterTemp°CElovaara, Samu
4SpeciesSpeciesElovaara, SamuFlow cytometry Accuri C6
5Uniform resource locator/link to referenceURL refElovaara, SamuWoRMS AphiaID
6ReplicateReplElovaara, Samu
7DATE/TIMEDate/TimeElovaara, SamuGeocode – experiment
8MicrophytoplanktonMicrophytopl#/lElovaara, SamuFlow cytometry Accuri C6
9MicrophytoplanktonMicrophytopl#/lElovaara, SamuFlow cytometry Accuri C6low chlorophyll a cells
10MicrophytoplanktonMicrophytoplankton%Elovaara, SamuFlow cytometry Accuri C6cells with intact membranes
11Absorption coefficient, 230 nmac2301/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
12Absorption coefficient, 254 nmac2541/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
13Absorption coefficient, 275 nmac2751/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
14Absorption coefficient, 295 nmac2951/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
15Absorption coefficient, 300 nmac3001/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
16Absorption coefficient, 350 nmac3501/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
17Absorption coefficient, 355 nmac3551/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
18Absorption coefficient, 375 nmac3751/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
19Absorption coefficient, 400 nmac4001/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
20Absorption coefficient, 440 nmac4401/mElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
21Spectral slope, 275-295 nmSpSl 275-295 nm1/µmElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
22Spectral slope, 350-400 nmSpSl 350-400 nm1/µmElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
23Slope ratioSRElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
24Spectral slope, 300-650 nmSpSl 300-650 nm1/µmElovaara, SamuSpectrophotometer UV/VIS (Shimadzu 2401PC)
25Fluorescence, peak TF peak TRUElovaara, SamuVarian Cary Eclipse fluorometer (Agilent)
26Fluorescence, peak AF peak ARUElovaara, SamuVarian Cary Eclipse fluorometer (Agilent)
27Fluorescence, peak MF peak MRUElovaara, SamuVarian Cary Eclipse fluorometer (Agilent)
28Fluorescence, peak CF peak CRUElovaara, SamuVarian Cary Eclipse fluorometer (Agilent)
29Fluorescence indexFIElovaara, SamuVarian Cary Eclipse fluorometer (Agilent)
30Humification indexHIXElovaara, SamuVarian Cary Eclipse fluorometer (Agilent)
31Biological indexBIXElovaara, SamuVarian Cary Eclipse fluorometer (Agilent)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3974 data points

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