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Ultrastructure of hepatocytes in golden ide (Leuciscus idus melanotus L.; Cyprinidae: Teleostei) during thermal adaptation

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Summary

The morphological alterations of hepatocytes of golden ide, Leuciscus idus melanotus, following adaptation to low and high temperatures (14 and 28°C) were investigated by means of light and electron microscopy. The temperature-dependent behaviour of peroxisomes was visualized cytochemically with the alkaline diaminobenzidine medium; the morphological studies were supplemented by the biochemical determination of catalase activity.

Cold adaptation of ide hepatocytes is manifested by proliferation and stacking of endoplasmic reticulum, an enhanced secretory activity of Golgi fields and a higher number of peroxisomes as compared with the warmadapted animals. The latter organelles are characterized by a marked heterogeneity in size, shape and catalase activity, and by a more intimate association with mitochondria and endoplasmic reticulum. The occurrence of small peroxisomal profiles is restricted to lower temperature. Catalase activity can be shown both cytochemically and biochemically to increase during cold adaptation.

Whereas the number of mitochondria seems to be unaffected by thermal adaptation, stacking of mitochondria as well as the formation of intramitochondrial membrane piles indicate cold-adaptive processes.

A feature typical of warm-adaptation is the formation of membrane-glycogen complexes, which may represent the morphological expression of enhanced carbohydrate metabolism documented in a decreased storage of glycogen at 28°C. At 28°C lipid is the predominant storage product.

These findings indicate that fish liver is well-suited to serve as a model for the analysis of the interaction of environmental temperature conditions and hepatic morphology.

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  1. Department of Zoology I, University of Heidelberg, Heidelberg, Germany

    Thomas Braunbeck & Volker Storch

  2. Department of Anatomy II, University of Heidelberg, Heidelberg, Germany

    Karin Gorgas & Alfred Völkl

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  1. Thomas Braunbeck
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Braunbeck, T., Gorgas, K., Storch, V. et al. Ultrastructure of hepatocytes in golden ide (Leuciscus idus melanotus L.; Cyprinidae: Teleostei) during thermal adaptation. Anat Embryol 175, 303–313 (1987). https://doi.org/10.1007/BF00309844

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