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Experience with the iodine-123 and technetium-99m labelled anti-granulocyte antibody MAb47: a comparison of labelling methods

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Abstract

Four different methods of radiolabelling the anti-granulocyte monoclonal antibody MAW were compared and their influence on diagnostic value studied. The best clinical images were obtained following labelling with iodine-123 by the Iodogen method and direct labelling with technetium-99m after tris-(carboxyethyl)-phosphine treatment of MAW to achieve disulphide bridge reduction.99mTc labelling using a specific ligand (MAb47-mtp), or a second method involving direct reduction with mercaptoethanol, led to an increased background activity in clinical studies, thus impeding the diagnosis of chronic disease. Fresh infections were clearly localized by all four preparations. The elimination of the activity from the blood was slower in the case of the iodinated MAb47, while the collected urine samples showed an excrection of about 10% of the injected activity per day independent of the labelling method. The results in terms of sensitivity and specificity were rather similar for all labelling methods and ranged from 90% to 99%.

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Author notes

  1. Adrian Ammann

    Present address: EAWAG, Swiss Federal Institute for Environmental Science and Technology, Dübendorf, Switzerland

Authors and Affiliations

  1. Division of Radiopharmacy, Paul Scherrer Institute, CH-5232, Villigen PSI, Switzerland

    Peter Bläuenstein, Peter Hasler, Adrian Ammann, Ilse Novak-Hofer, Alan Smith & P. August Schubiger

  2. Institute of Nuclear Medicine, Kantonsspital Aarau, Switzerland

    Johannes T. Locher & Klaus Seybold

  3. Institute of Nuclear Medicine, Charles University, Prague, Czech Republic

    Helena Koprivova

  4. National FJC Research Institute for Radiobiology and Hygiene, Budapest, Hungary

    Gyözö A. Janoki

  5. Institute of Nuclear Medicine, Kantonsspital Basel, Switzerland

    Helmut R. Mäcke

Authors
  1. Peter Bläuenstein
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  2. Johannes T. Locher
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  5. Gyözö A. Janoki
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  6. Helmut R. Mäcke
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  7. Peter Hasler
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  9. Ilse Novak-Hofer
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  10. Alan Smith
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  11. P. August Schubiger
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Bläuenstein, P., Locher, J.T., Seybold, K. et al. Experience with the iodine-123 and technetium-99m labelled anti-granulocyte antibody MAb47: a comparison of labelling methods. Eur J Nucl Med 22, 690–698 (1995). https://doi.org/10.1007/BF01254573

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