Summary
Biochemical, molecular and morphological markers have been used to monitor the segregation of alleles at major gene loci in microspore-derived lines of four spring barley crosses and their parents. Significant deviations from the expected Mendelian ratios were observed for four of the ten markers studied in the cross. Distorted ratios were associated with loci located on chromosomes 4H and 6H. The differential transmission of alleles was in favour of the responsive parent (Blenheim) used in the anther culture studies. For the α-Amy-1 locus on chromosome 6H, the preferential transmission of Blenheim alleles was most pronounced in the haploid regenerants that were colchicine treated. These results are discussed in relation to the genetic control of androgenetic response in barley and with respect to the exploitation of another culture in barley improvement.
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References
Agache S, Bachelier B, De Buyser J, Henry Y, Snape JW (1989) Genetical analysis of anther culture response in wheat using aneuploid, chromosome substitution and translocation lines. Theor Appl Genet 77:7–11
Bullock WP, Baenziger PS, Schaeffer GW, Bottino PJ (1982) Anther culture of wheat (Triticum aestivum L.) F1s and their reciprocal crosses. Theor Appl Genet 62:155–159
Choo TM, Reinbergs E, Kasha KJ (1985) Use of haploids in breeding barley. Plant Breed Rev 3:219–252
Corduan G (1975) Regeneration of anther-derived plants of Hyoscyamus niger L. Planta 127:27–36
Ellis THN, Goldsborough PB, Castleton JA (1983) Transcription and methylation of flax rDNA. Nucleic Acids Res 11:3047–3064
Finnie SJ, Powell W, Dyer AF (1989) The effect of carbohydrate composition and concentration on anther culture response in barley (Hordeum vulgare L.). Plant Breed 103:110–118
Forde BG, Kreis M, Williamson MS, Fry RP, Pywell J, Shewry PR, Bunce N, Miflin BJ (1985) Short tandem repeats shared by B-and C-hordein cDNAs suggest a common evolutionary origin for two groups of cereal storage protein genes. EMBO J 4:9–15
Foroughi-Wehr B, Friedt W (1984) Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Theor Appl Genet 67:377–387
Guiderdoni E, Glaszmann JC, Courtois B (1989) Segregation of 12 isozyme genes among doubled haploid lines derived from a Japonica x Indica cross of rice (Oryza sativa L.). Euphytica 42:45–53
Hayward MD, Olesen A, Due IK, Jenkins R, Morris P (1990) Segregation of isozyme marker loci amongst androgenetic plants of Lolium perenne L. Plant Breed 104:68–71
Kahler AL, Allard RW (1970) Genetics of isozyme variants in barley. I. Esterases. Crop Sci 10:444–448
Knudsen S, Due IK, Andersen SB (1989) Components of response in barley anther culture. Plant Breed 103:241–246
Lazar MD, Chen THH, Scores GJ, Kartha KK (1987) Immature embryo and anther culture of chromosome addition lines of rye in Chinese Spring wheat. Plant Sci 51:77–81
Liu CJ, Gale MD (1989) Ibf-1 (iodine binding factor), a highly variable marker system in the Triticeae. Theor Appl Genet 77:233–240
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Miah MAA, Earle ED, Khush GS (1985) Inheritance of callus formation ability in anther culture of rice Oryza sativa L. Theor Appl Genet 70:113–116
Murray M, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325
Nakata K (1971) Competition among pollen grains for haploid tobacco plant formation by anther culture. I. Analysis with leaf color character. Jpn J Breed 21:29–34
Nakata K, Kurihara T (1972) Competition among pollen grains for haploid tobacco plant formation by anther culture. II. Analysis with resistance to tobacco mosaic virus (TMV) and wild fire diseases, leaf color, and leaf-base shape characters. Jpn J Breed 22:92–98
Nielsen G, Frydenberg O (1971) Chromosome localisation of the esterase loci Est-1 and Est-2 in barley by means of trisomics. Hereditas 67:152–154
Oram RN, Doll H, Køie B (1975) Genetics of two storage protein variants in barley. Hereditas 80:53–58
Orton TJ, Browers MA (1985) Segregation of genetic markers among plants regenerated from cultured anthers of broccoli (Brassica oleracea var. italica). Theor Appl Genet 69:637–643
Pedersen S (1988) Pollen competition in barley. Hereditas 109:75–81
Powell W (1988) Diallel analysis of barley anther culture response. Genome 30:152–157
Powell W (1990) Environmental and genetical aspects of pollen embryogenesis. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 12. Springer, Berlin Heidelberg New York pp 45–65
Powell W, Borrino EM, Allison MJ, Griffiths DW, Asher MJC, Dunwell JM (1986) Genetical analysis of microspore-derived plants of barley (Hordeum vulgare). Theor Appl Genet 72:619–626
Powell W, Ellis RP, Macaulay M, McNicol J, Forster BP (1990) The effect of selection for protein and isozyme loci on quantitative traits in a doubled haploid population of barley. Heredity 65: 115–122
Powling A, Islang AKMR, Shepherd KW (1981) Isozymes in wheat-barley hybrid derivative lines. Biochem Genet 19:237–264
Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet 75:286–290
Shewry PR, Faulks AJ, Pickering RA, Jones IT, Finch RA, Miflin BJ (1980) The genetic analysis of barley storage proteins. Heredity 44:383–389
Siebel J, Pauls KP (1989) A comparison of anther and microspore culture as a breeding tool in Brassica napus. Theor Appl Genet 78:473–479
Snape JW, Simpson E, Parker BB, Friedt W, Foroughi-Wehr B (1986) Criteria for the selection and use of doubled haploid systems in cereal breeding programmes. In: Horn W, Jensen CJ, Odenbach W, Scheider O (eds) Genetic manipulation in plant breeding. Berlin, de Gruyter, pp 217–229
Thompson DM, Powell W, Forster BP (1990) Use of isoelectric focusing in barley varietal identification. Ann appl Biol (in press)
Zamir D, Tadmor Y (1986) Unequal segregation of nuclear genes in plants. Bot Gaz 147[3]:355–358
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Communicated by J.W. Snape
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Thompson, D.M., Chalmers, K., Waugh, R. et al. The inheritance of genetic markers in microspore-derived plants of barley Hordeum vulgare L.. Theoret. Appl. Genetics 81, 487–492 (1991). https://doi.org/10.1007/BF00219438
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DOI: https://doi.org/10.1007/BF00219438