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Establishment of primary cell cultures using short-term trypsinization of organ fragments

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Journal of tissue culture methods

Summary

A method for increasing surface adhesion of organ fragments, thus facilitating initiation of primary cultures, is described. The method involves treatment of tissue fragments with trypsin and has proven useful with tissues from several species.

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V. References

  1. Noga, E. J. Studies on channel catfish virus passaged in cell cultures of the walking catfish. Florida Atlantic Univeristy; 1977. Thesis. 15–18.

  2. Merchant, D. J.; Kahn, R. H.; Murphy, W. H. Handbook of cell and organ culture. Minneapolis, Minnesota: Burgess Publishing Co., 1964: 255–257.

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Authors and Affiliations

  1. Department of Biological Sciences, Florida Atlantic University, 33432, Boca Raton, Florida, USA

    Edward J. Noga

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  1. Edward J. Noga
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Noga, E.J. Establishment of primary cell cultures using short-term trypsinization of organ fragments. Journal of Tissue Culture Methods 6, 55–56 (1980). https://doi.org/10.1007/BF01666016

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  • DOI: https://doi.org/10.1007/BF01666016

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