Abstract
An efficient method was developed for radioiodination of melatonin with 125I. Chloramine-T and iodogen were used as oxidizing agents. The radioiodination was carried out via electrophilic substitution of the H atom with the I+ cation. Indirect iodination using Bolton–Hunter reagent was also performed. The influence exerted on the radiochemical yield by the melatonin concentration, pH of the reaction mixture, type of oxidizing agent, and reaction time was studied. The shelf life and specific activity of the product were determined. The radiochemical yield and product purity were determined by HPLC and paper electrophoresis. The immunoreactivity of the tracer was checked using its specific antibody. Specific and sensitive polyclonal antimelatonin was prepared using two New Zealand rabbits, which were injected with 5-methoxy-N-acetyltryptamine–bovine serum albumin conjugate as immunogen. The tracer prepared by the indirect method with iodogen had high specific activity, high yield, and long shelf life; it was also more selective in the melatonin assessment using radioimmunoassay technique.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hunter, W.M. and Greenwood, F.C., Nature, 1962, vol. 194, pp. 495–496.
Fraker, P.J. and Speck, J.C., Biochem. Biophys. Res. Commun., 1971, vol. 80, pp. 849–857.
Al-Aama, T., Brymer, C., Gutmanis, I., et al., Int. J. Geriatr. Psychiatry, 2011, vol. 26, no. 7, pp. 687–694.
Alstadhaug, K.B., Odeh, F., Salvesen, R., and Bekkelund, S.I., Neurology, 2010, vol. 75, no. 17, pp. 1527–1532.
Altun, A. and Ugur-Altun, B., Int. J. Clin. Pract., 2007, vol. 61, no. 5, pp. 835–845.
Arendt, J., New Engl. J. Med., 2000, vol. 343, no. 15, pp. 1114–1116.
Barcelo, E., Endocrine-Related Cancer, 2003, vol. 10, pp. 153–159.
Dominguez-Rodriguez, A., Expert Opin. Investig. Drugs, 2012, vol. 21, no. 11, pp. 1593–1596.
Dominguez-Rodriguez, A., Abreu-Gonzalez, P., and Avanzas, P., Front Biosci. (Landmark Ed.)., 2012, vol. 17, pp. 2433–2441.
Di, W.-L., Kadva, A., Djahanbakhch, O., and Silman, R., Clin. Chem., 1998, vol. 44, no. 2, pp. 304–310.
Grota, L.J., Snieckus, V., De Silva, S.O., and Brown, G.M., Can. J. Biochem. Cell Biol., 1983, vol. 61, pp. 1096–1101.
Ebeid, N.H., Shafik, H., Allan, K.F., and Ayobb, S.M., J. Isot. Radiat. Res., 2015, vol. 47, no. 1, pp. 111–123.
Hempen, C. and Karst, U., Anal. Bioanal. Chem., 2006, vol. 384, pp. 572–583.
Bolton, A.E. and Hunter, W.M., Biochem. J., 1973, vol. 133, pp. 529–539.
Manz, B., Seidel, A., Alexander, H., et al., J. Clin. Chem. Clin. Biochem., 1989, vol. 27, pp. 797–802.
Vakkuri, O., Leppaluoto, J., and Vuolteenaho, O., Acta Endocrinol. (Copenhagen), 1984, vol. 106, pp. 152–157.
Welp, A., Manz, B., and Peschke, E., J. Immunol. Meth., 2010, vol. 358, pp. 1–8.
Vakkuri, O., Lämsä, E., Rahkamaa, E., et al., Anal. Biochem., 1984, vol. 142, no. 2, pp. 284–289.
Mehany, N.L., El-Kolaly, M.T., Ayyoub, S.M., and Hassan, S.E.M., J. Radioanal. Nucl. Chem., 2005, vol. 265, no. 1, pp. 61–71.
Pillai, M.R.A. and Bhandarkar, S.D., Radioimmunoassay Principles and Practice, Trembay, Mumbai: Isotope Division, Bhabha Atomic Energy Centre, 1998, 2nd ed., p. 89.
Sallam, Kh.M., El-Bayoumy, A.S.A., and Mehany, N.L., J. Radioanal. Nucl. Chem., 2016, vol. 307, pp. 1375–1383.
Sanad, M.H., Sallam, Kh.M., Marzook, F.A., and Abd-Elhaliem, S.M., J. Label. Compd. Radiopharm., 2016, vol. 59, no. 12, pp. 484–491.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Russian in Radiokhimiya, 2017, Vol. 59, No. 6, pp. 562–566.
The text was submitted by the authors in English.
Rights and permissions
About this article
Cite this article
Sallam, K.M., El-Bayoumy, A.S.A. & Farouk, N. Radiolabeling of melatonin using different oxidizing agents for immunoassay purpose. Radiochemistry 59, 639–644 (2017). https://doi.org/10.1134/S10663622170600133
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S10663622170600133