Abstract
Two strains of Pseudo-nitzschia fukuyoi isolated from Vietnamese waters produce domoic acid, a toxin responsible for amnesic shellfish poisoning. Species identification was based on detailed morphological observation using a transmission electron microscope and also molecular data on large subunit (LSU) and the second internal transcribed spacer (ITS2) with NCBI nucleotide Blast (blastn). Toxin productivity of the two strains was confirmed and their range were 3.85–4.54 pg/cell by analyses using LC–MS/MS. This is the first report of occurrence of P. fukuyoi in Vietnamese waters, and the first confirmation of productivity of domoic acid of the species.
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Lim HC, Su SNP, Mohamed-Ali H, Kotaki Y, Leaw CP, Lim PT (2010) Toxicity of diatom Pseudo-nitzschia (Bacillariophyceae) analyzed using high performance liquid chromatography (HPLC). J Sci Technol Trop 6:S116–S119
Lim HC, Leaw CP, Su SNP, Teng ST, Usup G, Noor NM, Lundholm N, Kotaki Y, Lim PT (2012) Morphology and molecular characterization of Pseudo-nitzschia (Bacillariophyceae) from Malaysian Borneo, including the new species Pseudo-nitzschiacircumpora sp. nov. J Phycol 48(5):1232–1247
Lim HC, Teng ST, Leaw CP, Lim PT (2013) Three novel species in the Pseudo-nitzschia pseudo delicatissima complex: P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia. J Phycol 49(5):902–916
Teng ST, Leaw CP, Lim HC, Lim PT (2013) The genus Pseudo-nitzschia (Bacillariophyceae) in Malaysia, including new records and a keyto species inferred from morphology-based phylogeny. Bot Mar 56:375–398
Teng ST, Lim HC, Lim PT, Dao VH, Bates SS, Leaw CP (2014) Pseudo-nitzschiakodamae sp. nov. (Bacillariophyceae), a toxigenic species from the Strait of Malacca, Malaysia. Harmful Algae 34:17–28. doi:10.1016/j.hal.2014.02.005
Skov J, Ton TP, Do TBL (2004) Bacillariophyceae. In: Potentially toxic microalgae of Vietnamese waters. In: Larsen J, Nguyen NL (eds) Opera Botanica, vol 140. Council for Nordic Publications in Botany, Copenhagen, pp 23–51
Doan NH, Tin NT, Anh NTM, Lam NN (2013) Species composition and cell density variation of diatoms Pseudo-nitzschia spp. in coastal waters of Khanh Hoa, Vietnam. In: Bui HL et al (eds) Proceedings of the international conference on “Bien Dong 2012”, 12–14 September, 2012 (in Vietnamese with English abstract), pp 159–268
Dao VH, Lim PT, Ky PX, Takata Y, Teng ST, Omura T, Fukuyo Y, Kodama M (2014) Diatom Pseudo-nitzschia cf. caciantha (Bacillariophyceae), the most likely source of domoic acid contamination in the thorny oyster Spondylusversicolor Schreibers 1793 in Nha Phu Bay, Khanh Hoa Province, Vietnam. Asian Fish Sci 27:16–29
DaoVH, Takata Y, Omura T, Sato S, Fukuyo Y, Kodama M (2009) Seasonal variation of domoic acid in Spondylus versicolor in association with that in plankton samples in Nha Phu Bay, Khanh Hoa, Vietnam. Fish Sci 75:507–512
Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds) Culture of marine invertebrate animals. Plenum Press, New York, pp 26–60
Bates SS, de Freltas ASW, Milley JE, Pocklington R, Quilliam MA, Smith JC, Worms J (1991) Controls on domoic acid production by the diatom Nitzschiapungens f. multiseries in culture nutrients and irradiance. Can J Fish Aquat Sci 48:1136–1144
Lundholm N, Bates SS, Baugh KA, Bill BD, Connell LB, Léger C, Trainer VL (2012) Cryptic and pseudo-cryptic diversity in diatoms-with descriptions of Pseudo-nitzschiahasleana sp. nov. and P. fryxelliana sp. nov. J Phycol 48(2):436–454
Bargu S, Koray T, Lundholm N (2002) First report of Pseudo-nitzschia calliantha Lundholm, Moestrup and Hasle 2003, a new potentially toxic species from Turkish Coast. J Fish Aqua Sci 19(3–4):479–483
White T, Bruns T, Lee S, Taylor J, Innis M, Gelfand D, Shinsky J, White T (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA et al (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322
Scholin CA, Herzog M, Sogin M, Anderson DM (1994) Identification of group-and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragment of the LSU rRNA gene. J Phycol 30(6):999–1011
Orive E, Pérez-Aicua L, David H, García-Etxebarria K, Laza-Martínez A, Seoane S, Miguel I (2013) The genus Pseudo-nitzschia (Bacillariophyceae) in a temperate estuary with description of two new species: Pseudo-nitzschia plurisecta sp. nov. and Pseudo-nitzschia abrensis sp. nov. J Phycol 49:1192–1206
Lundholm N, Moestrup Ø, Hasle GR, Hoef-Emden K (2003) A study of the Pseudo-nitzschia pseudodelicatissima/cuspidata complex (Bacillariophyceae): what is P. pseudodelicatissima? J Phycol 39:797–813
Amato A, Montresor M (2008) Morphology, phylogeny, and sexual cycle of Pseudo-nitzschia mannii sp. nov. (Bacillariophyceae): a pseudocryptic species within the P. pseudodelicatissima complex. Phycologia 47:487–497
Lelong AL, Hégaret HH, Soudant P, Bates SS (2012) Pseudo-nitzschia (Bacillariophyceae) species, domoic acid and amnesic shellfish poisoning: revisiting previous paradigms. Phycologia 51:168–216
Fernandes LF, Hubbard KA, Richlen M, Smith J, Bates SS, Ehrman J, Léger C, Mafra LL Jr, Kulis D, Quilliam M, Erdner D, Libera K, McCauley L, Anderson D (2014) Diversity and toxicity of the diatom Pseudo-nitzschia Peragallo in the Gulf of Maine, Northwestern Atlantic Ocean. Deep Sea Res II 103:139–162
Trainer VL, Bates SS, Lundholm N, Thessen AE, Cochlan WP, Adams NG, Trick CG (2012) Pseudo-nitzschia physiological ecology, phylogeny, toxicity, monitoring and impacts on ecosystem health. Harmful Algae 14:271–300
Acknowledgments
This study was funded by project 106.99-2010.22—NAFOSTED, Vietnam. The authors wish to express the deep thank to Dr. Stephen S. Bates (Fisheries and Oceans Canada, Gulf Fisheries Centre, Canada) for the help to improve the manuscript, both in scientific ideas and English correction.
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Dao, H.V., Phan, V.B., Teng, S.T. et al. Pseudo-nitzschia fukuyoi (Bacillariophyceae), a domoic acid-producing species from Nha Phu Bay, Khanh Hoa Province, Vietnam. Fish Sci 81, 533–539 (2015). https://doi.org/10.1007/s12562-015-0864-9
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DOI: https://doi.org/10.1007/s12562-015-0864-9