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Antimicrobial and Antibiofilm Effect of Inulin-Type Fructans, Used in Synbiotic Combination with Lactobacillus spp. Against Candida albicans

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Abstract

There is great interest in the search for new alternatives to antimicrobial drugs, and the use of prebiotics and probiotics is a promising approach to this problem. This study aimed to assess the effect of inulin-type fructans, used in synbiotic combinations with Lactobacillus paracasei or Lactobacillus plantarum, on the production of short-chain fatty acids and antimicrobial activity against Candida albicans. The inhibition assay using the L. paracasei and L. plantarum supernatants resulting from the metabolization of inulin-type fructans displayed growth inhibition and antibiofilm formation against C. albicans. Inhibition occurred at concentrations of 12.5, 25, and 50% of the L. paracasei supernatant and at a concentration of 50% of the L. plantarum supernatant. The analysis of short-chain fatty acids by gas chromatography showed that lactic acid was the dominating produced metabolite. However, acetic, propionic, and butyric acids were also detected in supernatants from both probiotics. Therefore, the synbiotic formulation of L. paracasei or L. plantarum in the presence of inulin-type fructans constitutes with anticandidal effect is a possible option to produce antifungal drugs or antimicrobial compounds.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgments

The authors would like to thank the financial support granted by CONACYT (CVU number 662891).

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Authors and Affiliations

  1. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas No. 800, col Colinas de la Normal, C.P. 44270, Guadalajara, Jalisco, Mexico

    Ricardo García-Gamboa, Miguel Ángel Domínguez-Simi, Jorge Bravo-Madrigal & Marisela González-Avila

  2. Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Av. General Ramón Corona No. 2514, Col. Nuevo México, Jalisco, C.P. 45138, Zapopan, Mexico

    Ricardo García-Gamboa & Misael Sebastián Gradilla-Hernández

  3. Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO), Av. de Cataluña 21, 46020, València, Spain

    Andrés Moya

Authors
  1. Ricardo García-Gamboa
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Correspondence to Marisela González-Avila.

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García-Gamboa, R., Domínguez-Simi, M.Á., Gradilla-Hernández, M.S. et al. Antimicrobial and Antibiofilm Effect of Inulin-Type Fructans, Used in Synbiotic Combination with Lactobacillus spp. Against Candida albicans. Plant Foods Hum Nutr 77, 212–219 (2022). https://doi.org/10.1007/s11130-022-00966-3

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