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Molecular diversity of endosymbiotic Nephroselmis (Nephroselmidophyceae) in Hatena arenicola (Katablepharidophycota)

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Abstract

Hatena arenicola (Katablepharidophycota) is a single-celled eukaryote that temporarily possesses a chlorophyte alga of the genus Nephroselmis as an intracellular symbiont. In the present study, we investigated the molecular diversity of the endosymbiont Nephroselmis in a natural population of the host H. arenicola. We sequenced the host’s 18S rRNA gene and the endosymbiont’s plastid-encoded 16S rRNA gene. The results indicated that almost identical strains of the host harbored at least three distinct strains of the algal endosymbiont affiliated to the clade Nephroselmis rotunda. This finding supports our previous hypothesis that H. arenicola and its symbiotic alga are in an early stage of secondary endosymbiosis.

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Acknowledgments

This work was supported by JSPS KAKENHI Grant Number 22657024 to T. N. and I. I., Grant Number 23117004 to I. I., and the NEXT program grant to Y. H. We thank Prof. Suda (University of the Ryukyus) for depositing the N. clavistella culture in NIES.

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Authors and Affiliations

  1. National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan

    Haruyo Yamaguchi & Masanobu Kawachi

  2. Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8572, Japan

    Takeshi Nakayama & Isao Inouye

  3. Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8550, Japan

    Yuichi Hongoh

Authors
  1. Haruyo Yamaguchi
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  2. Takeshi Nakayama
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  3. Yuichi Hongoh
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  4. Masanobu Kawachi
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  5. Isao Inouye
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Correspondence to Haruyo Yamaguchi.

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Yamaguchi, H., Nakayama, T., Hongoh, Y. et al. Molecular diversity of endosymbiotic Nephroselmis (Nephroselmidophyceae) in Hatena arenicola (Katablepharidophycota). J Plant Res 127, 241–247 (2014). https://doi.org/10.1007/s10265-013-0591-1

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