Summary
Magnetic beads coupled with secondary monoclonal antibodies were used to remove cells expressing the common acute lymphoblastic leukemia antigen by adsorption. The influences of the bead-to-cell ratio, temperature and the amout of antibodies used on the effectiveness of the immunomagnetic cell isolation were studied.
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References
Bell GI, (1978)Science, 200, 618–627
Gaudernack G, Leivestad T, Ugelstad J, and Thorsby E, (1986)J. Immunol. Methods, 90, 179–187
Hammer DA, Linderman JJ, Graves DJ, and Lauffenburger A. (1988)Biotechnol. Prog., 3, 189–204
Kvalheim G, Fodstad O, Pihl A, Nustad K, Pharo A, Ugelstad J, and Funderud S, (1987)Cancer Res., 47, 846–851
Lea T, Smeland E, Funderud S, Vartdal F, Davies C, Beiske K, and Ugelstad J, (1986)Scand. J. Immunol., 23, 509–519
Minowada J, Oshimura M, Abe S, Greaves MF, Janossy G, and Sandberg AA, (1978)Proc. Am. Assoc. Cancer. Res., 19, 109
Nilsson H, Johansson C, and Scheynius A, (1987)J. Immunol. Methods, 105, 165–169
Treleaven JG, Gibson FM, Ugelstad J, Rembaum A, Philip T, Caine GD, and Kemshead JT, (1984)Lancet, 1, 70–73
Yeh HM, Su JL, Shiao KI, Lin SH, Chuang MW, Tzeng CH, and Chen PM, (1988)Chin. Med. J. (Taipei), 42, 417–424
Ugelstad J, Rembaum A, Kemshead JT, Nustad K, Funderud S, and Schmid R, (1984) inMicrospheres and Drug Therapy, Pharmaceut-ical, Immunological and Medical Aspects, Davis SS, Illum L, McVie J, and Tomlinson E, eds., pp. 365–381, Elsevier, Amsterdam.
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Hsu, SC., Yeh, HM., Chu, IM. et al. Antibody-antigen mediated adsorption in the immunomagnetic cell isolation system. Biotechnol Tech 3, 257–260 (1989). https://doi.org/10.1007/BF01876060
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DOI: https://doi.org/10.1007/BF01876060