Leys, Sally P; Federwisch, Luisa (2024): Histological micrographs (Masson's trichrome stain) of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.966416, In: Federwisch, Luisa; Leys, Sally P; Janussen, Dorte; Linse, Katrin; Hentschel, Ute; Busch, Kathrin (2024): Images and microbiome data of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected during expeditions PS96 and JR17003a in the Weddell Sea, Antarctica [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.965868
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Abstract:
This image dataset provides histological micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Histological sections of 7-30 µm were stained in Masson's trichrome and images were captured on a Zeiss Axioskop 2 plus with a QiCam camera using Northern Eclipse software. Image files are labeled with expedition, station, sample ID, and magnification. Masson's trichrome highlights collagen in blue (especially spongin).
Three images of a calibration scale at 10x, 20x, and 40x magnification are included (see Further details link). To derive the scale for an image, open both the image and the corresponding calibration scale of the same magnification in e.g. ImageJ or Photoshop and draw a line across the calibration. Then pull that line over to the image as a layer. Each smallest mark equals 10 micrometers.
Keyword(s):
Further details:
Project(s):
Bentho-Pelagic Processes @ AWI (AWI_BPP)
Coverage:
Median Latitude: -63.826788 * Median Longitude: -58.016557 * South-bound Latitude: -64.057780 * West-bound Longitude: -58.477560 * North-bound Latitude: -63.615860 * East-bound Longitude: -57.501430
Date/Time Start: 2018-03-05T14:18:00 * Date/Time End: 2018-03-07T17:54:00
Minimum Elevation: -845.0 m * Maximum Elevation: -429.0 m
Event(s):
JR17003A_38 * Latitude Start: -64.053780 * Longitude Start: -58.476010 * Latitude End: -64.057780 * Longitude End: -58.477560 * Date/Time: 2018-03-05T14:18:00 * Elevation Start: -787.0 m * Elevation End: -740.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
JR17003A_46 * Latitude Start: -63.806030 * Longitude Start: -58.065230 * Latitude End: -63.810580 * Longitude End: -58.070640 * Date/Time: 2018-03-06T17:48:00 * Elevation Start: -845.0 m * Elevation End: -825.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
JR17003A_52 * Latitude Start: -63.615860 * Longitude Start: -57.501430 * Latitude End: -63.616700 * Longitude End: -57.508470 * Date/Time: 2018-03-07T17:54:00 * Elevation Start: -489.0 m * Elevation End: -429.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Federwisch, Luisa | |||
| 2 | Latitude of event | Latitude | Federwisch, Luisa | |||
| 3 | Longitude of event | Longitude | Federwisch, Luisa | |||
| 4 | Elevation of event | Elevation | m | Federwisch, Luisa | ||
| 5 | Sample ID | Sample ID | Federwisch, Luisa | |||
| 6 | Image number/name | Image no/name | Federwisch, Luisa | |||
| 7 | Magnification | Mag | Federwisch, Luisa | |||
| 8 | Image | IMAGE | Federwisch, Luisa | Histological sections stained with Masson's trichrome |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Basic curation (CurationLevelB)
Size:
524 data points