Citation:

PANGAEA.965093

Name: The effect of light intensity versus light dose on the photosynthetic performance of two strains of Ulva lacinulata
Published: 2024-01-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Daily light integral; Laboratory experiment; light intensity; PAM fluorometry; Photosynthetic parameters; Ulva

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Abstract:

The genus Ulva, described as a good source of antioxidants known for its antibacterial properties and associated with the capacity to adapt to different environments and high growth rates has justified the increasing interest in its large-scale production. While extensive research has been done on optimizing the extraction of Ulva's bioactive compounds, few studies were conducted on increasing or optimizing antioxidant activity of Ulva spp. during cultivation. Our study aimed to investigate an energy-saving cultivation method for optimizing Ulva lacinulata by testing the impact of light dose and light intensity on its antioxidant activity. Two geographically different strains (NE-Atlantic and Mediterranean) were observed for 5 days under two light intensities (70 or 185 µmol photons m-2 s-1) with the same light dose (4 mol photons m-2 d-1). Samples were collected at different times to evaluate the photosynthetic performance (using a Pulse Amplitude Modulated fluorometer). A strain-dependent response was observed between the NE-Atlantic strain and the Mediterranean strain, suggesting that light dose may significantly affect antioxidant activity in certain Ulva spp.

Keyword(s):

Daily light integral; Laboratory experiment; light intensity; PAM fluorometry; Photosynthetic parameters; Ulva

Related to:

Cardoso, Isabel; Hofmann, Laurie C; Meiβner, Anneke (submitted): The effect of light intensity versus light dose on the antioxidant activity of two strains of Ulva lacinulata.

Funding:

Federal Ministry of Food and Agriculture (BMEL), grant/award no. 281DL02B20 : Verbundprojekt: Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel (MakPakScaleUp) - Teilprojekt B

Coverage:

Median Latitude: 39.980497 * Median Longitude: 6.872087 * South-bound Latitude: 39.394977 * West-bound Longitude: -9.213583 * North-bound Latitude: 40.566018 * East-bound Longitude: 22.957756

Date/Time Start: 2017-01-01T00:00:00 * Date/Time End: 2021-04-01T12:00:00

Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 0 m

Event(s):

Ulva_Mediterranean * Latitude: 40.566018 * Longitude: 22.957756 * Date/Time: 2017-01-01T00:00:00 * Comment: Sampling date is not correct. Only the year is known.

Ulva_NE-Atlantic * Latitude: 39.394977 * Longitude: -9.213583 * Date/Time: 2021-04-01T12:00:00 * Comment: Species were collected during low tide in a Lagoon were there was no water (at that specific time)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Hofmann, Laurie C
2	Latitude of event	Latitude		Hofmann, Laurie C
3	Longitude of event	Longitude		Hofmann, Laurie C
4	Date/Time of event	Date/Time		Hofmann, Laurie C
5	DEPTH, water	Depth water	m	Hofmann, Laurie C		Geocode
6	Type of study	Study type		Hofmann, Laurie C
7	Laboratory	Lab		Hofmann, Laurie C
8	Latitude 2	Latitude 2		Hofmann, Laurie C		of laboratory
9	Longitude 2	Longitude 2		Hofmann, Laurie C		of laboratory
10	Date/time start, experiment	Date/time start exp		Hofmann, Laurie C
11	Date/time end, experiment	Date/time end exp		Hofmann, Laurie C
12	Analysis date/time, experiment	Date/time analysis exp		Hofmann, Laurie C	Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany)	of PAM measurements
13	Species, unique identification	Species UID		Hofmann, Laurie C
14	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Hofmann, Laurie C
15	Species, unique identification (URI)	Species UID (URI)		Hofmann, Laurie C
16	Collection	Collection		Hofmann, Laurie C		Collection number
17	Accession number, genetics	Access no gen		Steinhagen, Sophie		Molecular analysis with the Invisorb Spin Plant Mini Kit (Stratec); gel electrophoresis and subsequently purified using the QIAquick PCR Purification Kit (Qiagen); Sanger sequencing was performed by Eurofins Genomics (Konstanz, Germany); Sequence reads were assembled in sequencher (version 4.1.4, Gene Codes Corporation). BLAST function in GenBank, identifications via the specimens' tufA sequences were made.
18	Experiment duration	Exp duration	h	Hofmann, Laurie C
19	Replicate	Repl		Hofmann, Laurie C
20	Treatment: temperature	T:temp	°C	Hofmann, Laurie C	Digital thermometer
21	Treatment: light:dark cycle	T:L:D	hh:hh	Hofmann, Laurie C
22	Treatment: light condition	T:light		Hofmann, Laurie C		""SL"" - Saturating Light, ""C"" - Control
23	Treatment: light intensity	T:Io	µmol/m²/s	Hofmann, Laurie C	Light meter, LI-COR Biosciences GmbH, LI-250A
24	Treatment: nutrients	T:nutrients		Hofmann, Laurie C		Fertilizer: Blaukorn (COMPO SANA®, Germany)
25	Treatment: nutrients	T:nutrients		Hofmann, Laurie C		Concentration in ml/l
26	Replicate	Repl		Hofmann, Laurie C		In each Ulva piece that was analyzed with the PAM, 3 random points of the piece were selected and considered a sub-replicate of that piece. From each beaker 1 piece was analyzed at hours 0, 48 and 120 (n=3). 2 treatments (HL and Control), 3 replicates each, 3 sub-replicates, 3 time points for measuring.
27	Treatment: number of light pulses	T:light pulse	#	Hofmann, Laurie C
28	Radiation, photosynthetically active, wavelength	PAR	nm	Hofmann, Laurie C
29	Fluorescence, maximum	Fm		Hofmann, Laurie C	Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany)
30	Fluorescence, maximum, without dark adaptation	Fm'		Hofmann, Laurie C	Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany)
31	Fluorescence, minimum	Fo		Hofmann, Laurie C	Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany)
32	Effective quantum yield of photosystem II	Y(II)		Hofmann, Laurie C	Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany)
33	Electron transport rate	ETR	µmol e/m²/s	Hofmann, Laurie C	Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany)	Rapid light curves were analyzed. Electron transport rate (ETR) was measured and calculated using the equation reported in Heinz Walz GmbH, 2019

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

28080 data points

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