Two-dimensional distributions of calcium, phosphorus, strontium, and zinc in regenerating scales of carp, Cyprinus carpio, were observed over a period of 90 days by utilizing synchrotron radiation-excited X-ray fluorescence (SRXRF) imaging. On the 15th day, these elements were highly accumulated in the part that is exposed to the ambient water, and the accumulated amount decreased linearly toward the basal edge through the focal area of the scale. As the regeneration progressed, this characteristic pattern changed such that the amount of each element in the rim part, which was exposed to the ambient water, obviously increased, while the amount in the basal edge decreased. However, the relative amounts of phosphorus, strontium, and zinc with respect to calcium in the regenerating scale remained almost constant during the regeneration. In addition, it was found that the diameter of, and the number of ridges in, the regenerating scales increased rapidly during the first 15 days, and increased at a slower pace later on.