Expression of Matrix Metalloproteinase, a Disintegrin and Metalloproteinase, and Tissue Inhibitor of Metalloproteinase in Human Chondrosarcoma

Hideyuki Sugita; Shunzo Osaka; Masato Toriyama; Eiji Osaka; Yukihiro Yoshida; Junnosuke Ryu; Makoto Sano; Masahiko Sugitani; Norimichi Nemoto

doi:10.1267/ahc.37.319

RAPID COMMUNICATION

Expression of Matrix Metalloproteinase, a Disintegrin and Metalloproteinase, and Tissue Inhibitor of Metalloproteinase in Human Chondrosarcoma

Hideyuki Sugita, Shunzo Osaka, Masato Toriyama, Eiji Osaka, Yukihiro Yoshida, Junnosuke Ryu, Makoto Sano, Masahiko Sugitani, Norimichi Nemoto

Author information

Hideyuki Sugita
Department of Orthopaedic Surgery, Nihon University School of Medicine
Shunzo Osaka
Department of Orthopaedic Surgery, Nihon University School of Medicine
Masato Toriyama
Department of Orthopaedic Surgery, Nihon University School of Medicine
Eiji Osaka
Department of Orthopaedic Surgery, Nihon University School of Medicine
Yukihiro Yoshida
Department of Orthopaedic Surgery, Nihon University School of Medicine
Junnosuke Ryu
Department of Orthopaedic Surgery, Nihon University School of Medicine
Makoto Sano
Department of Pathology, Nihon University School of Medicine
Masahiko Sugitani
Department of Pathology, Nihon University School of Medicine
Norimichi Nemoto
Department of Pathology, Nihon University School of Medicine

Keywords: chondrosarcoma, matrix metalloproteinase, tissue inhibitor of metalloproteinase, a disintegrin and metalloproteinase, immunohistochemistry

JOURNAL FREE ACCESS

2004 Volume 37 Issue 5 Pages 319-323

DOI https://doi.org/10.1267/ahc.37.319

Details

Abstract

Malignant degree of human chondrosarcoma can be difficult to determine using only histological findings. We therefore assessed expression of matrix metalloproteinases (MMPs), a disintegrin and metalloproteinase (ADAM) with thrombospondin motifs (ADAMTSs) and tissue inhibitor of metalloproteinases (TIMPs) in chondrosarcoma and ascertained relationships to histological degree of malignancy. As methods, in 28 chondrosarcoma cases, immunostaining was performed using antibodies against MMP 2, 3, 7, 9, 13, ADAMTS 4, 5 and TIMP 1, 2, 3. Chondrosarcoma were classified into groups of 7, 15 and 6 cases based on histologically malignant grade I, II and III, respectively. All target proteins were expressed in chondrosarcoma. Positive correlations (p<0.05) existed between immunostaining scores and histological grades for all proteins except MMP 9, with strong correlations (p<0.01) for MMPs 2, 3 and 13, both ADAMTSs and all 3 TIMPs. It was concluded that these proteins could be used to indicate degree of malignancy in human chondrosarcoma.

Corresponding author

Register with J-STAGE for free!