Abstract
Eight pepper genotypes (Capsicum sp., Capsicum annun L., C. chinense Jacq, and C. baccatum L. var. umbilicatum) were assayed for total phenolics, anthocyanins, carotenoids, vitamin C, and total antioxidant activity in order to determine their bioactive compound profile and to establish the correlations between these compounds and their antioxidant activity. The genotype IAN 186311 (C. chinense Jacq.) showed the highest total antioxidant activity and the highest concentrations of phenolic compounds. As for the content of carotenoids, the genotype with the highest concentration was IAN 186324 (Capsicum sp.), and the genotype IAN 186305 (C. baccatum L. var. umbilicatum) showed the highest content of vitamin C and anthocyanins. Terefore, it can be concluded that the Capsicum sp. genotypes analyzed demonstrated a rather high diversity of bioactive compounds that should be further explored given the benefits they can provide to consumer health.
Similar content being viewed by others
References
Alvarez-Parrilla E, De La Rosa LA, Amarowicz R, Shahidi F (2011) Antioxidant activity of fresh and processed Jalapeño and Serrano peppers. J Agric Food Chem 59:163–173
AOAC (1984). Official methods of analysis of the association of official analytical chemists. 14 ed. Arlington, VA, USA
Arnnok P, Ruangviriyachai C, Mahachai R, Techawongstien S, Chanthai S (2012) Determination of total phenolics and the anthocyanin contents in the pericarp of hot chilli pepper (Capsicum annuum L.). Int Food Res J 19:235–243
Basiouny FM, Biswas PK (1981) Ascorbic acid, pigments and mineral element contents associated with growth and development of pimiento pepper. Proc Florida State Hortic Soc 94:268–269
Brand-Wiliams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Food Sci Technol 28:25–30
Cardoso LM, Leite JPV, Peluzio MC (2011) Efeitos biológicos das antocinaninas no processo aterosclerótico. Rev Colomb Ciências Químico-Farmacêutica 40:16–138
Castañeda-Ovando A, Pacheco-Hernández ML, Páez-Hernández ME, Rodríguez JA, Galán-Vidal CA (2009) Chemical studies of anthocyanins: a review. Food Chem 113:859–871
Chuah AM, Lee YC, Yamaguchi T, Takamura H, Yin LJ, Matoba T (2008) Effect of cooking on the antioxidant properties of coloured peppers. Food Chem 111:20–28
Collera-Zúñiga O, Jiménez FG, Gordillo RM (2005) Comparative study of carotenoid composition in three mexican varieties of Capsicum annuum L. Food Chem 90:109–114
Conforti F, Statti GA, Menichini F (2007) Chemical and biological variability of hot pepper fruits (Capsicum annuum var. acuminatum L.) in relation to maturity stage. Food Chem 102:1096–1104
Dastmalchi K, Flores G, Petrova V, Pedraza-Penalosa P, Kennelly EJ (2011) Edible neotropical blueberries: antioxidant and compositional fingerprint analysis. J Agric Food Chem 59:3020–3026
Deepa N, Kaur C, George B, Singh B, Kapoor HC (2007) Antioxidant constituents in some sweet pepper (Capsicum annuum L.) genotypes during maturity. LWT-Food Sci Technol 40:121–129
Deli J, Molnar P, Matus Z, Toth G (2001) Carotenoid composition in the fruits of red paprika (Capsicum annuum var. lycopersiciforme rubrum) during ripening; biosynthesis of carotenoids in red parika. J Agric Food Chem 49:1517–1523
do Rufino MSM, Alves RE, Brito ES, de Pérez-Jiménez J, Saura-Calixto F, Mancini-Filho J (2010) Bioactive compounds and antioxidant capacities of 18 non-traditional tropical fruits from Brazil. Food Chem 121:996–1002
Edge R, Mcgarvey DJ, Truscott TG (1997) The carotenoids as anti-oxidants – a review. J Photochem Photobiol B Biol 41:189–200
Finco FDBA, Kammerer DR, Reinhold Carle R, Wen-Hsin Tseng WH, BöSer S, Graeve L (2012) Antioxidant activity and characterization of phenolic compounds from bacaba (Oenocarpus bacaba Mart.) fruit by HPLC-DAD-MS. J Agric Food Chem 60:7665–7673
Francis FJ (1989) Food colorants: anthocyanins. Crit Rev Food Sci Nutr 28:273–314
Fuleki T, Francis FJ (1968) Quantitative methods for anthocyanins.1. Extraction and determination of total anthocyanin in Cranberries. J Food Sci 33:72–77
Garcia-Alonso M, Minihane AM, Rimbach G, Rivas-Gonzalo JC, Tereza SP (2009) Red wine anthocyanins are rapidly absorbed in humans and affect monocyte chemoattractant protein 1 levels and antioxidant capacity of plasma. J Nut Biochem 20:521
Godoy HT, Rodriguez-Amaya DB (1994) Occurrence of cis-isomers of provitamin A in Brazilian fruits. J Agric Food Chem 42:1306–1313
Guilland JC, Lequeu B (1995) As vitaminas: do nutriente ao medicamento. Santos, São Paulo, Original Les Vitamines Tec & Doc; Lavoisier: Paris
Guzman I, Hamby S, Romero J, Bosland PW, O’Connell MA (2010) Variability of carotenoid biosynthesis in orange colored Capsicum spp. Plant Sci 179:49–59
Hart DJ, Scott KJ (1995) Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK. Food Chem 54:101–111
Howard LR, Talcott ST, Brenes CH, Villalon B (2000) Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum spp.) as influenced by maturity. J Agric Food Chem 48:1713–1720
Kong J, Chia L, Goh N, Chia T, Brouillard R (2003) Analysis and biological activities of anthocyanins. Phytochemistry 64:923–933
Larrauri JA, Rupérez P, Saura-Calixto F (1997) Effect of drying temperature on the stability of polyphenols and antioxidant activity of red grape pomace peels. J Agric Food Chem 45:1390–1393
Lees DH, Francis FJ (1972) Standardization of pigment analyses in cranberries. HortSci 7:83–84
Mares-Perlman TL, Ficek A, Klein RG, Millen AE, Hankinson SE (2002) The body of evidence to support a protective role for lutein and zeaxanthin in delaying chronic disease. Overview. J Nutr 132:518S–524S
Marín A, Ferreres F, Tomás-Barberán FA, Gil MI (2004) Characterization and quantification of antioxidant constituents of sweet pepper (Capsicum annuum L.). J Agric Food Chem 52:3861–3869
Menichini F, Tundis R, Bonesi M, Loizzo MR, Conforti F, Statti G, De Cindio B, Houghton PJ, Menichini F (2009) The influence of fruit ripening on the phytochemical content and biological activity of Capsicum chinense Jacq. cv Habanero. Food Chem 114:553–560
Mercadante AZ, Rodriguez-Amaya DB (1998) Effects of ripening, cultivar differences, and processing on the carotenoid composition of mango. J Agric Food Chem 46:128–130
Miller NJ, Diplock AT, Rice-Evans C, Davies MJ, Gopinathan V, Milner A (1993) A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84:407–412
Ogiso Y, Hosoda-Yabe R, Kawamoto Y, Kawamoto T, Kato K, Yabe T (2008) An antioxidante of dried chilli pepper maintained its activity through postharvest ripening for 18 months. Biosci Biotechnol Biochem 2:3297–3300
Palevitch D, Cracker LEJ (1995) Herbs, spices. Med Plants 3:55–83
Prior RL, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53:4290–4302
Reifschneider FJB (2000) Capsicum: pimentas e pimentões. Embrapa Comunicação para Transferência de Tecnologia, Brasília, 113p
Rockenbach II, Rodrigues E, Gonzaga LV, Caliari V, Genovese MI, Gonçalves AESS, Fett R (2011) Phenolic compounds content and antioxidant activity in pomace from selected red grapes (Vitis vinifera L. and Vitis labrusca L.) widely produced in Brazil. Food Chem 127:174–179
Sadilova E, Stintzing FC, Carle R (2006) Anthocyanins, colour and antioxidante properties of eggplant (Solanum melongena L.) and violet pepper (Capsicum annuum L.) peel extracts. Z Naturforsch C 61:527–535
SAS for Windows, versão 8.0 (199\9) SAS® institute inc., SAS user guide. Statistical Analysis System Institute, Carry
Singleton VL, Rossi JA Jr (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 6:144–158
Topuz A, Ozdemir F (2007) Assessment of carotenoids, capsaicinoids and ascorbic acid composition of some selected pepper cultivars (Capsicum annuum L.) grown in Turkey. J Food Compos Anal 20:596–602
Wahyuni Y, Ballester AR, Sudarmonowati E, Bino RJ, Bovy AG (2011) Variation in health-related compounds and implications for breeding. Phytochemistry 72:1358–1370
Xia M, Ling W, Zhu H, Ma J, Wang Q, Hou M, Tang Z, Guo H, Liu C, Ye Q (2009) Anthocyanin attenuates CD40-mediated endothelial cell activation ans apoptosis by inhibiting CD40-induced MAPK activation. Atherosclerosis 202:41
Young A, Lowe GM (2001) Antioxidant and prooxidant proprieties of carotenoids. Arch Biochem Biophys 385:20–27
Zanatta CF, Mercadante AZ (2007) Carotenoid composition from the Brazilian tropical fruit camu-camu (Myrciaria dubia). Food Chem 101:1526–1532
Zhuang Y, Chen L, Sun L, Cao J (2012) Bioactive characteristics and antioxidant activities of nine peppers. J Funct Foods 4:331–338
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Carvalho, A.V., de Andrade Mattietto, R., de Oliveira Rios, A. et al. Bioactive compounds and antioxidant activity of pepper (Capsicum sp.) genotypes. J Food Sci Technol 52, 7457–7464 (2015). https://doi.org/10.1007/s13197-015-1833-0
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13197-015-1833-0