Abstract
Stimulation of microbially induced calcium carbonate precipitation (MICCP) is likely to be influenced by calcium sources. In order to study such influences, we performed MICCP using Bacillus sp. CR2 in nutrient broth containing urea, supplemented with different calcium sources (calcium chloride, calcium oxide, calcium acetate and calcium nitrate). The experiment lasted 7 days, during which bacterial growth, urease activity, calcite production and pH were measured. Our results showed that calcium chloride is the better calcium source for MICCP process, since it provides higher urease activity and more calcite production. The influences of calcium sources on MICCP were further studied using Fourier transform-infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses. These analyses confirmed that the precipitate formed was CaCO3 and composed of predominantly calcite crystals with a little amount of aragonite and vaterite crystals. The maximum yield of calcite precipitation was achievable with calcium chloride followed by calcium nitrate as a calcium source. The results of present study may be applicable to media preparation during efficient MICCP process.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kang, C.H., Han, S-H., Shin, Y.J., Oh, S.J., & So, J.S. (2013). Applied Biochemistry and Biotechnology. doi: 10.1007/s12010-013-0626-z.
Achal, V., Mukherjee, A., & Reddy, M. S. (2011). Journal of Materials in Civil Engineering, 23, 730–734.
Ferris, F. G., Phoenix, V., Fujita, Y., & Smith, R. W. (2003). Geochimica et Cosmochimica Acta, 68, 1701–1710.
Fujita, Y., Redden, G. D., Ingram, J. C., Cortez, M. M., Ferris, G. F., & Smith, R. W. (2004). Geochimica et Cosmochimica Acta, 68, 3261–3270.
Hammes, F., Seka, A., Van Hege, K., Van De Wiele, T., Vanderdeelen, J., Siciliano, S. D., & Verstraete, W. (2003). Journal of Chemical Technology & Biotechnology, 78, 670–677.
Whiffin, V. A., Van Paassen, L. A., & Harkes, M. P. (2007). Geomicrobiology Journal, 24, 417–423.
De Muynck, W., Belie, N., & Verstraete, W. (2010). Ecological Engineering, 36, 118–136.
Ghosh, S., Biswas, M., Chattopadhyay, B. D., & Mandal, S. (2009). Cement and Concrete Research, 31, 93–98.
Sarayu, K., Iyer, N.R., & Ramachandra Murthy, A. (2014). Applied Biochemistry and Biotechnology. doi: 10.1007/s12010-013-0686-0.
Dhami, N.K., Reddy, M.S., & Mukherjee, A. (2014). Applied Biochemistry and Biotechnology. doi: 10.1007/s12010-013-0694-0.
Achal, V., & Pan, X. (2011). Current Microbiology, 62, 894–902.
Little, B., Wagner, P. A., & Lewandowski, Z. (1997). Review in Mineralogy, 35, 123–159.
Rodriguez-Navarro, C., Rodriguez-Gallego, M., Chekroun, K. B., & Gonzalez-Muñoz, M. T. (2003). Applied and Environmental Microbiology, 69, 2182–2193.
Stocks-Fischer, S., Galinat, J. K., & Bang, S. S. (1999). Soil Biology and Biochemistry, 31, 1563–1571.
Achal, V., Mukherjee, A., & Reddy, M. S. (2010). Industrial Biotechnology, 6, 170–174.
Achal, V., Pan, X., & Zhang, D. (2011). Ecological Engineering, 37, 1601–1605.
Shirakawa, M. A., Cincotto, M. A., Atencio, D., Gaylarde, C. C., & John, V. M. (2011). Brazilian Journal of Microbiology, 42, 499–507.
Beveridge, T. J., & Fyfe, W. S. (1985). Canadian Journal of Earth Science, 22, 1893–1898.
Achal, V., Mukherjee, A., Basu, P. C., & Reddy, M. S. (2009). Journal of Industrial Microbiology and Biotechnology, 36, 981–988.
Dittrich, M., Müller, B., Mavrocordatos, D., & Wehrli, B. (2003). Acta Hydrochimica Hydrobiology, 31, 162–169.
De Muynck, W., Verbeken, K., Belie, N., & Verstraete, W. (2010). Ecological Engineering, 36, 99–111.
Achal, V., Mukherjee, A., Basu, P. C., & Reddy, M. S. (2009). Journal of Industrial Microbiology and Biotechnology, 36, 433–438.
Lee, Y. N. (2003). The Journal of Microbiology, 41, 345–348.
Fujita, Y., Taylor, J. L., Gresham, T. L., Delwiche, M. E., Colwell, F. S., Mcling, T. L., Petzke, L. M., & Smith, R. W. (2008). Environmental Science & Technology, 42, 3025–3032.
Stumm, W., & Morgan, J. J. (1981). Aquatic chemistry. New York: Wiley-Interscience.
McConnaughey, T. A., & Whelan, F. F. (1997). Earth Science Review, 42, 95–117.
El Kahoui, R., Adolphe, J., & Daudon, M. (2000). Microbes and Environment, 15, 161–171.
Gayathri, S., Lakshminarayanan, R., Weaver, J. C., Morse, D. E., Kini, R. M., & Valiyaveettil, S. (2007). Chemistry - A European Journal, 13, 3262–3268.
Oh, S. E., Hassan, S. H. A., & Joo, J. H. (2009). World Journal of Microbiology and Biotechnology, 25, 1771–1778.
Nassrallah-Aboukaïs, N., Jacquemin, J., Decarne, C., Abi-Aad, E., Lamonier, J. F., & Aboukaïs, A. (2003). Journal of Thermal Analysis and Calorimetry, 74, 21–27.
Kemperl, J., & Maček, J. (2009). International Journal of Mineral Processing, 93, 84–88.
House, W. A. (1987). Journal of Colloid and Interface Science, 119, 507–511.
Chafetz, H. S., Patrick, F. R., & Utech, N. M. (1991). Sedimentology, 38, 107–126.
Boquet, E., Boronat, A., & Ramos-Cormenzana, A. (1973). Nature, 246, 527–528.
Urzi, C., Garcia-Valles, M. T., Vendrell, M., & Pernice, A. (1999). Geomicrobiology Journal, 16, 39–54.
Lippmann, F. (1973). Sedimentary carbonate minerals. NewYork: Springer-Verlag.
Acknowledgements
This work was supported by National Natural Science Foundation of China (U1120302, 41072195 and 41350110533). We are thankful to the editor and reviewers for their constructive comments for the improvement of the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Achal, V., Pan, X. Influence of Calcium Sources on Microbially Induced Calcium Carbonate Precipitation by Bacillus sp. CR2. Appl Biochem Biotechnol 173, 307–317 (2014). https://doi.org/10.1007/s12010-014-0842-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-014-0842-1