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Extraction of Pectin from Satsuma Mandarin Orange Peels by Combining Pressurized Carbon Dioxide and Deionized Water: a Green Chemistry Method

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Abstract

Pectic oligosaccharides have vital properties that are dependent on their molecular assemblies, such as the degree of esterification (DE) and branched side chains. The molecular structures are affected by the extraction solvents and conditions. In this study, we used pressurized carbon dioxide treatment (CT) as a novel method for pectin extraction from fresh and ripe orange peels and compared the total yield, DE, molecular weight, and monosaccharide compositions of the product obtained with those of products obtained using conventional extraction methods, such as heating with HCl or chelating with sodium hexametaphosphate (SHMP). CT provided a yield of approximately 3.8%, which was less than that of HCl (9.9%/dry weight). However, DE in the CT extract was approximately 94%, which was higher than that of HCl (75%) and SHMP (59%). The molecular weights of pectins in the CT extract appeared to be higher than that of HCl, except at a pressure of 2 MPa. β-Galacturonic acid was the major monosaccharide in pectin extracted by HCl and SHMP, whereas that was decreased in CT extract from fresh oranges. The results showed that extraction with CT contributed to a high DE pectin yield and diverse pectin molecular structures compared to conventional methods, although its application resulted in a lower overall pectin yield. These findings suggest the possibility of manufacturing oligosaccharides with novel properties.

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Data Availability (data transparency)

All data obtained through this study are presented in this manuscript and can be provided to researchers who demand them.

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References

  • Al-Assaf, S., Phillips, G. O., & Williams, P. A. (2006). Controlling the molecular structure of food hydrocolloids. Food Hydrocolloids, 20(2-3), 369–377.

    Article  CAS  Google Scholar 

  • Albersheim, P., Darvill, A. G., O’Neill, M. A., Schols, H. A., & Voragen, A. G. J. (1996). An hypothesis: The same six polysaccharides are components of the primary cell walls of all higher plants. In J. Visser & A. G. J. Voragen (Eds.), Pectins and pectinases (pp. 47–55). Elsevier Science.

  • Babbar, N., Van Roy, S., Wijnants, M., Dejonghe, W., Caligiani, A., Sforza, S., et al. (2016). Effect of extraction conditions on the saccharide (neutral and acidic) composition of the crude pectic extract from various agro-industrial residues. Journal of Agricultural and Food Chemistry, 64(1), 268–276.

    Article  CAS  Google Scholar 

  • Bagherian, H., Ashtiani, F. Z., Fouladitajar, A., & Mohtashamy, M. (2011). Comparisons between conventional, microwave- and ultrasound-assisted methods for extraction of pectin from grapefruit. Chemical Engineering and Processing, 50(11-12), 1237–1243.

    Article  CAS  Google Scholar 

  • Bélafi-Bakó, K., Cserjési, P., Beszédes, S., Csanádi, Z., & Hodúr, C. (2012). Berry pectins: Microwave-assisted extraction and rheological properties. Food and Bioprocess Technology, 5, 1100–1105.

    Article  Google Scholar 

  • Bitter, T., & Muir, H. M. (1962). A modified uronic acid carbazole reaction. Analytical Biochemistry, 4(4), 330–334.

    Article  CAS  Google Scholar 

  • Carpita, N. C., & Gibeaut, D. M. (1993). Structural models of primary cell walls in flowering plants: Consistency of molecular structure with the physical properties of the walls during growth. Plant Journal, 3(1), 1–30.

    Article  CAS  Google Scholar 

  • De Oliveira, C. F., Gurak, P. D., Cladera-Olivera, F., Marczak, L. D. F., & Karwe, M. (2016). Combined effect of high-pressure and conventional heating on pectin extraction from passion fruit peel. Food and Bioprocess Technology, 9(6), 1021–1030.

    Article  Google Scholar 

  • De Vries, J. A., Voragen, A. G. J., Rombouts, F. M., & Pilnik, W. (1981). Extraction and purification of pectins from alcohol insoluble solids from ripe and unripe apples. Carbohydrate Polymers, 1(2), 117–127.

    Article  Google Scholar 

  • El-Nawawi, S. A., & Shehata, F. R. (1987). Extraction of pectin from Egyptian orange peel. Factors affecting the extraction. Biological Wastes, 20(4), 281–290.

    Article  CAS  Google Scholar 

  • Fuchigami, M. (1983). Effect of pH on heat degradation of pectin. Journal of Japan Society of Nutrition and Food Sciences, 36(4), 294–298 (in Japanese).

    Article  CAS  Google Scholar 

  • Fuchigami, M. (2007). Heating of vegetables and pectic substances. Journal of Cookery Science of Japan, 40(1), 1–9 (in Japanese).

    Google Scholar 

  • Hosseini, S. S., Khodaiyan, F., & Yarmand, M. S. (2016). Aqueous extraction of pectin from sour orange peel and its preliminary physicochemical properties. International Journal of Biological Macromolecules, 82, 920–926.

    Article  CAS  Google Scholar 

  • Ichida, J. (2005). Production and physiological functions of apple pectin and pectic oligosaccharides. The Journal of Japan Mibyou System Association, 11(2), 304–308.

    Google Scholar 

  • Jenkins, D. J. A., Leeds, A. R., Newton, C., & Cummings, J. H. (1975). Effect of pectin, guar gum, and wheat fibre on serum-cholesterol. The Lancet, 305(7916), 1116–1117.

    Article  Google Scholar 

  • Kar, F., & Arslan, N. (1999). Effect of temperature and concentration on viscosity of orange peel pectin solutions and intrinsic viscosity–molecular weight relationship. Carbohydrate Polymers, 40(4), 277–284.

    Article  CAS  Google Scholar 

  • Kastner, H., Einhorn-Stoll, U., & Senge, B. (2012). Structure formation in sugar containing pectin gels-influence of Ca2+ on the gelation of low-methoxylated pectin at acidic pH. Food Hydrocolloids, 27(1), 42–49.

    Article  CAS  Google Scholar 

  • Kaya, M., Sousa, A. G., Crepeau, M., Srensen, S. O., & Ralet, M. (2014). Characterization of citrus pectin samples extracted under different conditions: Influence of acid type and pH of extraction. Annals of Botany, 114(6), 1319–1326.

    Article  CAS  Google Scholar 

  • Kertesz, Z. I. (1951). The pectic substances. Interscience Publishers.

  • Kurita, O., Fujiwara, T., & Yamazaki, E. (2008). Characterization of the pectin extracted from citrus peel in the presence of citric acid. Carbohydrate Polymers, 74(3), 725–730.

    Article  CAS  Google Scholar 

  • Liu, L., Cao, J., Huang, J., Cai, Y., & Yao, J. (2010). Extraction of pectins with different degrees of esterification from mulberry branch bark. Bioresource Technology, 101(9), 3268–3273.

    Article  CAS  Google Scholar 

  • Liu, F., Wang, Y., Bi, X., Guo, X., Fu, S., & Liao, X. (2013). Comparison of microbial inactivation and rheological characteristics of mango pulp after high hydrostatic pressure treatment and high temperature short time treatment. Food and Bioprocess Technology, 6, 2675–2684.

    Google Scholar 

  • Manabe, T. (2001). Pectin. Saiwai Shobo.

  • Masmoudi, M., Besbes, S., Chaabouni, M., Robert, C., Paquot, M., Blecker, C., & Attia, H. (2008). Optimization of pectin extraction from lemon by-product with acidified date juice using response surface methodology. Carbohydrate Polymers, 74(2), 185–192.

    Article  CAS  Google Scholar 

  • Methacanon, P., Krongsin, J., & Gamonpilas, C. (2014). Pomelo (Citrus maxima) pectin: Effects of extraction parameters and its properties. Food Hydrocolloids, 35, 383–391.

    Article  CAS  Google Scholar 

  • Meyssami, B., Balaban, M. O., & Teixeira, A. A. (1992). Prediction of pH in model systems pressurized with carbon dioxide. Biotechnology Progress, 8(2), 149–154.

    Article  CAS  Google Scholar 

  • Muñoz-Almagro, N., Valadez-Carmona, L., Mendiola, J. A., Ibáñez, E., & Villamiel, M. (2019). Structural characterisation of pectin obtained from cacao pod husk. Comparison of conventional and subcritical water extraction. Carbohydrate Polymers, 217, 69–78.

    Article  Google Scholar 

  • Muralikrishna, G. (1994). Characterization of pectic polysaccharides from pulse husks. Food Chemistry, 50(1), 87–89.

    Article  CAS  Google Scholar 

  • Ridley, B. L., O'Neill, M. A., & Mohnen, D. (2001). Pectins: Structure, biosynthesis, and oligogalacturonide-related signaling. Phytochemistry, 57(6), 929–967.

    Article  CAS  Google Scholar 

  • Saeed, A. R. (1975). Characterization of pectic substances in mango marc. Journal of Food Science, 40(1), 205–206.

    Article  CAS  Google Scholar 

  • Sayah, M. Y., Chabir, R., Benyahia, H., Kandri, Y. R., Chahdi, F. O., et al. (2016). Yield, esterification degree and molecular weight evaluation of pectins isolated from orange and grapefruit peels under different conditions. PLoS ONE, e0161751.

  • Sherry, C. L., Kim, S. S., Dilger, R. N., Bauer, L. L., Moon, M. L., Tapping, R. I., Fahey Jr., G. C., Tappenden, K. A., & Freund, G. G. (2010). Sickness behavior induced by endotoxin can be mitigated by the dietary soluble fiber, pectin, through up-regulation of IL-4 and Th2 polarization. Brain, Behavior, and Immunity, 24(4), 631–640.

    Article  CAS  Google Scholar 

  • Shpigelman, A., Kyomugasho, C., Christiaens, S., & Van Loey, A. M. (2015). The effect of high-pressure homogenization on pectin: Importance of pectin source and pH. Food Hydrocolloids, 43, 189–198.

    Article  CAS  Google Scholar 

  • Strydom, D. J. (1994). Chromatographic separation of 1-phenyl-3-methyl-5-pyrazolone-derivatized neutral, acidic and basic aldoses. Journal of Chromatography A, 678(1), 17–23.

    Article  CAS  Google Scholar 

  • Tarutani, T., & Manbe, M. (1963). Study on production of low methoxyl pectin. Journal of Food Science and Technology, 10(8), 316–320.

    Article  CAS  Google Scholar 

  • Vriesmann, L. C., Teófilo, R. F., & Petkowicz, C. L. O. (2011). Optimization of nitric acid-mediated extraction of pectin from cacao pod husks (Theobroma cacao L.) using response surface methodology. Carbohydrate Polymers, 84(4), 1230–1236.

    Article  CAS  Google Scholar 

  • Vriesmann, L. C., Teófilo, R. F., & Petkowicz, C. L. O. (2012). Extraction and characterization of pectin from cacao pod husks (Theobroma cacao L.) with citric acid. LWT - Food Science and Technology, 49(1), 108–116.

    Article  CAS  Google Scholar 

  • Willats, W. G. T., McCartney, L., Mackie, W., & Knox, J. P. (2001). Pectin: Cell biology and prospects for functional analysis. Plant Molecular Biology, 47(1-2), 9–27.

    Article  CAS  Google Scholar 

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Funding

This work was supported by the Japan Society for the Promotion of Science (17K00818).

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Authors and Affiliations

  1. Faculty of Agriculture, Saga University, Saga, Japan

    Chiaki Tsuru & Ayana Umada

  2. Faculty of Agriculture, College of Natural Sciences, Institute of Education and Research, Saga University, Saga, Japan

    Seiji Noma, Mikihide Demura & Nobuyuki Hayashi

  3. The United Graduate School of Agricultural Sciences Kagoshima University, Kagoshima, Japan

    Seiji Noma & Nobuyuki Hayashi

Authors
  1. Chiaki Tsuru
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  2. Ayana Umada
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  3. Seiji Noma
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  4. Mikihide Demura
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  5. Nobuyuki Hayashi
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Correspondence to Seiji Noma.

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Tsuru, C., Umada, A., Noma, S. et al. Extraction of Pectin from Satsuma Mandarin Orange Peels by Combining Pressurized Carbon Dioxide and Deionized Water: a Green Chemistry Method. Food Bioprocess Technol 14, 1341–1348 (2021). https://doi.org/10.1007/s11947-021-02644-9

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