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Polyphasic identification and MAT1-2 isolates of Phyllosticta citricarpa in Cuba

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Abstract

Citrus black spot (CBS), caused by Phyllosticta citricarpa (McAlpine) van der Aa, is a disease that affects Citrus spp. causing production losses, high costs associated with management, and constraints on the fresh market trade. The present investigation aimed to confirm the identity of the CBS pathogen as P. citricarpa and determine its mating-type distribution in Cuba. Phyllosticta isolates were collected from major Cuban citrus-producing provinces (Matanzas, Cienfuegos, Camagüey, and Santiago de Cuba). P. citricarpa and the endophyte P. capitalensis (Henn.) were identified by polyphasic taxonomy, integrating morpho-physiological characteristics with the phylogenetic analysis of ITS, actA, LSU and tef1 loci. Mating-type (MAT) analysis revealed that only the MAT1-2 idiomorph of P. citricarpa is present in Cuba, providing evidence of an atypical CBS disease cycle. These results contribute to a better understanding of the epidemiology of CBS in Cuba and the improvement of CBS management.

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Data availability

The datasets generated during and/or analyzed during the current study are either included in this published article and its supplementary information files, or available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the Research and Technological Innovation Grant [2067], from Agricultural Business Group, Cuba; the Cuban National Project [3009] of the Animal and Plant Health Program; the BOF incoming mobility program, from Hasselt University, Belgium and CRDF-UF Research Agreement 18-006. We also thanks to L. Mieres, F. A. Díaz Leyva, V. Zamora, I. Peña, X. Ferriol, A. H. Vega, A. Fuentes, M. Almaguer, G. Recio, E. Canales and M. Rodríguez for their assistance with this research.

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Authors and Affiliations

  1. Research Institute for Tropical Fruit Crops, 7th Ave, 3005, Playa, Havana, Cuba

    Wendy Serra, María B. Lugo Álvarez, Amalia Sanz Llorente & Ana M. Manzano León

  2. Central Laboratory for Plant Quarantine, National Center for Plant Health, Plaza, Havana, Cuba

    Dariel García Rodríguez

  3. Citrus Company “Victoria de Girón”, Jagüey Grande, Matanzas, Cuba

    Eugenio Alonso-Oliva

  4. Centre for Innovation in the Agro-Environmental Sector, AGROINNOVA, University of Torino, Largo Braccini 2, 10095, Grugliasco, TO, Italy

    Vladimiro Guarnaccia

  5. Department of Agricultural, Forest and Food Sciences (DiSAFA), University of Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy

    Vladimiro Guarnaccia

  6. Citrus Research International, P.O. Box 28, Nelspruit, 1200, South Africa

    Elma Carstens

  7. Department of Plant Pathology, University of Florida, Gainesville, FL, USA

    Jeffrey A. Rollins

  8. Centre for Environmental Sciences, Hasselt University, Agoralaan, building D, B-3590, Diepenbeek, Belgium

    Sofie Thijs & Jaco Vangronsveld

  9. Faculty of Biology and Biotechnology, Department of Plant Physiology and Biophysics, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland

    Jaco Vangronsveld

Authors
  1. Wendy Serra
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  2. María B. Lugo Álvarez
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  3. Dariel García Rodríguez
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  4. Eugenio Alonso-Oliva
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  5. Amalia Sanz Llorente
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  6. Vladimiro Guarnaccia
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  7. Elma Carstens
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  8. Jeffrey A. Rollins
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  9. Sofie Thijs
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  10. Jaco Vangronsveld
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  11. Ana M. Manzano León
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Contributions

Wendy Serra, Eugenio Alonso-Oliva, Vladimiro Guarnaccia, Jeffrey A. Rollins, Sofie Thijs, Jaco Vangronsveld and Ana M. Manzano León designed the work. Ana M. Manzano León, Sofie Thijs and Jaco Vangronsveld coordinated the execution of the projects. Wendy Serra, María B. Lugo Álvarez, Dariel García Rodríguez, Amalia Sanz Llorente and Ana M. Manzano León performed the experiments. Wendy Serra, Eugenio Alonso-Oliva, Vladimiro Guarnaccia, Jeffrey A. Rollins, Elma Carstens, Sofie Thijs, Jaco Vangronsveld and Ana M. Manzano León interpreted the data, wrote and approved the final version of the manuscript.

Corresponding author

Correspondence to Ana M. Manzano León.

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Highlights

• Polyphasic taxonomy confirms the presence of Phyllosticta citricarpa and P. capitalensis in Cuba.

• The presence of only the MAT1-2 idiomorph in Cuba, provides evidence of an atypical CBS cycle.

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Serra, W., Lugo Álvarez, M.B., García Rodríguez, D. et al. Polyphasic identification and MAT1-2 isolates of Phyllosticta citricarpa in Cuba. Eur J Plant Pathol 162, 995–1003 (2022). https://doi.org/10.1007/s10658-021-02453-y

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