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Exosomal miR-214-3p as a potential novel biomarker for rhabdoid tumor of the kidney

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Abstract

Purpose

Rhabdoid tumor of the kidney (RTK) is a rare, highly aggressive pediatric renal tumor. No specific biomarkers are available for detection of RTK, and the initial differential diagnosis from other pediatric abdominal tumors, including neuroblastoma (NB), is difficult. Exosomal miRNAs are novel cancer biomarkers that can be detected in biological fluids. We explored candidate RTK-specific exosomal miRNAs as novel biomarkers of RTK.

Methods

Exosomal miRNAs were collected from conditioned media of human RTK-derived cell lines, a human embryonic renal cell line, and human NB-derived cell lines. miRNA sequencing (miRNA-Seq) was performed to detect candidate RTK-specific exosomal miRNAs. The exosomal miRNA expression in conditioned media of tumor cell lines and serum from RTK xenograft-bearing mice was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

Results

The expression of exosomal miR-214-3p detected by miRNA-Seq was highest in RTK-derived cell lines. Exosomal miR-214-3p expression level determined by qRT-PCR was significantly higher in RTK-derived cell lines than in the human embryonic renal cell line or NB-derived cell lines. Furthermore, the serum exosomal miR-214-3p expression level was significantly higher in RTK xenograft mice than controls.

Conclusion

Our data indicated that exosomal miR-214-3p has potential as a novel biomarker of RTK.

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Data availability

The datasets used and/or analyzed during the present study are available from the corresponding author upon reasonable request.

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Acknowledgements

We thank Ms. A. Oguni for her excellent technical support and Ms. E. Mizutani for her secretarial assistance. The present study was supported in part by JSPS KAKENHI Grant Number 17K11524 to SU.

Funding

The present study was supported in part by JSPS KAKENHI Grant Number 17K11524 to SU.

Author information

Author notes

  1. Bin Yamaoka and Eri Nagasaki-Maeoka contributed equally to this work.

Authors and Affiliations

  1. Department of Pediatric Surgery, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi, Tokyo, 173-8610, Japan

    Bin Yamaoka, Eri Nagasaki-Maeoka, Shota Uekusa, Ayano Hidaka, Kohei Hijikata, Reona Kato, Tsugumichi Koshinaga & Shuichiro Uehara

  2. Division of General Medicine, Department of Medicine, Nihon University School of Medicine, Itabashi, Tokyo, 173-0032, Japan

    Kyoko Fujiwara

  3. Department of Anatomy, Nihon University School of Dentistry, Chiyoda, Tokyo, 101-8310, Japan

    Kyoko Fujiwara

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  1. Bin Yamaoka
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Contributions

Shuichiro Uehara (SU) and Eri Nagasaki-Maeoka (ENM) planned the experiments. Bin Yamaoka (BY), ENM, Shota Uekusa, Ayano Hidaka, Kohei Hijikata, and Reona Kato performed the experiments. BY, ENM, Kyoko Fujiwara (KF), and SU interpreted experimental data. BY and ENM wrote the paper. SU, Tsugumichi Koshinaga, and KF revised the manuscript critically for important intellectual content. All authors have read and approved the final manuscript.

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Correspondence to Shuichiro Uehara.

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Yamaoka, B., Nagasaki-Maeoka, E., Uekusa, S. et al. Exosomal miR-214-3p as a potential novel biomarker for rhabdoid tumor of the kidney. Pediatr Surg Int 37, 1783–1790 (2021). https://doi.org/10.1007/s00383-021-04989-w

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