Streptococcus pneumoniae Cell-Wall-Localized Phosphoenolpyruvate Protein Phosphotransferase Can Function as an Adhesin: Identification of Its Host Target Molecules and Evaluation of Its Potential as a Vaccine
Fig 9
Effects of target-derived peptides on nasopharyngeal and lung colonization and survival in mice.
Strain WU2 cells were pretreated with peptide and then inoculated IN into adult CBA/Nxid mice. As a control, another group of mice was injected with bacteria treated ex vivo for 1 h with PBS alone. Mice were euthanized 3, 24 or 48 h later. The nasopharynx and lungs were excised, homogenized and plated onto blood agar plates for counting. A) Eps 1 (14.7 μM), nasopharynx, 3 h (P < 0.001); B) Eps 1 (14.7 μm), lung, 3 h (P < 0.0001); C) Eps 1 (14.7 μm), nasopharynx, 24 h (P < 0.0001); D) Eps 1 (14.7 μm) lung, 24 h (P < 0.0001); E) BMPER, nasopharynx, 48 h at 7 and 14 μM (P < 0.0001); F) BMPER, lung, 48 h at 7 and 14 μM (P < 0.0001); G) PCDH19, nasopharynx, 48 h at 14.7 and 17.4 μM (P < 0.001); H) PCDH19, lung, 48 h (P < 0.0001).