Persistent ER Stress Induces the Spliced Leader RNA Silencing Pathway (SLS), Leading to Programmed Cell Death in Trypanosoma brucei
Figure 8
Cytoplasmic calcium concentration is elevated in SLS induced cells.
A. Uninduced cells or SEC63 cells silenced for 20, 42 or 48 hours were treated with 1 µM Fluo-4-AM as described in Materials and Methods. The cells were then analyzed by FACS. B. Calcium increase upon DTT treatment. Cytoplasmic calcium was measured in cells treated with DTT for 0, 1 or 4 hours as described in Panel A. C. Calcium increase upon DTT treatment in calcium-free medium. Cytoplasmic calcium was measured in cells untreated or treated with DTT in medium lacking or containing 1 mM EGTA. Cells were treated with DTT for 4 hours. The symbols indicate the different treatments.