Streptococcus pneumoniae Cell-Wall-Localized Phosphoenolpyruvate Protein Phosphotransferase Can Function as an Adhesin: Identification of Its Host Target Molecules and Evaluation of Its Potential as a Vaccine
Fig 8
Inhibition of adhesion of S. pneumoniae cells to A549 cells by target-derived peptides.
S. pneumoniae cells were treated for 1 h with peptide and added to A549 cells for 1 h; non-adherent bacteria were removed; and cells were detached with trypsin and plated onto blood agar plates for counting. A) BMPER, WU2 (P < 0.0001, r = −0.887); B) BMPER, 3.8DW (P < 0.0001, r = −883); C) Col VIIα WU2 (P < 0.0001, r = −1); D) Col VIIα, 3.8DW (P < 0.0001, r = −0.886); E) MMRN1, WU2 (P < 0.0001, r = −1); F) MMRN1, 3.8DW (P < 0.0001, r = −0.821); G) PCDH 19, WU2 (P < 0.0001, r = −0.829); H) PCDH 19, 3.8DW (P < 0.0001, r = −0.754); I) Int β4, WU2 (P < 0.0001, r = −0.5619); J) Int β4, 3.8DW (P < 0.0001, r = −0.967); K) No significant inhibition was observed with a scrambled peptide (K scrambled peptide, P = 0.9981). Experiments were performed in triplicates and repeated at least 3 times. Values are means±SD. *Student's t-test p<0.05.