Solanum lycopersicum GOLDEN 2-LIKE 2 transcription factor affects fruit quality in a light- and auxin-dependent manner
Fig 8
Transcript profile of tocopherol-related encoding genes in fruits from SlGLK2 overexpressing line.
Heatmap representation of the mRNA abundance of tocopherol-related encoding genes in the pedicellar portion of immature green (IG5) and ripe (Br+5) fruits of untransformed genotypes (SlGLK2 and au-Slglk2) and transformed au-Slglk2 SlGLK2-overexpressing L2 line. Values are normalised against the respective au-Slglk2 sample. Coloured squares indicate statistically significant differences compared to au-Slglk2 (P < 0.05). Values represent mean of at least three biological replicates. Relative transcript values are described in S6 Table. Methylerythritol phosphate pathway (orange), shikimate pathway (red), chlorophyll metabolism (green), phytol recycling (purple) and tocopherol biosynthesis (blue). Dotted lines indicate that intermediate steps were omitted. Enzymes: DXS: 1-DEOXY-D-XYLULOSE-5-P SYNTHASE; HPPD2: 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE (2); GGDR: GERANYLGERANYL DIPHOSPHATE REDUCTASE; VTE1: TOCOPHEROL CYCLASE; VTE2: HOMOGENTISATE PHYTYL TRANSFERASE; VTE3: 2,3-DIMETHYL-5-PHYTYLQUINOL METHYL TRANSFERASE; VTE4: TOCOPHEROL Γ -METHYL TRANSFERASE; VTE5: PHYTOL KINASE; VTE6: PHYTYL-PHOSPHATE KINASE; PPH: PHEOPHYTINASE; PPHL1: PHEOPHYTINASE LIKE-1; CHLG: CHLOROPHYLL SYNTHASE. Metabolites: G3P: glyceraldehyde 3-phosphate; DXP: 1-deoxy-D-xylulose-5P; GGDP: geranylgeranyl-2P; PDP: phytyl diphosphate; HGA: homogentisate; HPP: hydroxyphenylpyruvate; PEP: phosphoenolpyruvate; E4P: erythrose 4-phosphate; MPBQ: 2-methyl-6-phytylquinol; DMBQ: 2,3-dimethyl-5-phytylquinol; PP: phytyl phosphate.