NCX1 represents an ionic Na+ sensing mechanism in macrophages
Fig 8
Slc8a1 silencing decreases Na+ influx and Ca2+ efflux.
(A) Slc8a1 and (B) membranous NCX1 expression in LPS-stimulated ns or Slc8a1-specific siRNA–treated BMDMs after 4 h (means ± SD; n = 8; Mann–Whitney test; *p < 0.05). (C) Relative [Na+]i levels of ns siRNA and Slc8a1 siRNA–treated BMDMs exposed to LPS ± HS at t = 10 s (means ± SD; n = 5–7). (D) Relative [Ca2+]i levels in ns siRNA and Slc8a1 siRNA–treated BMDMs exposed to LPS ± HS at t = 10 s (means ± SD; n = 6–11). For numerical raw data, please see S1 Data. For raw immunoblots, please see S1 Blots. BMDM, bone marrow–derived MΦ; [Ca2+]i, intracellular Ca2+ in situ; HS, high salt; LPS, lipopolysaccharide; [Na+]i, intracellular Na+ in situ; ns, nonsilencing; NCX, Na+/Ca2+ exchanger; siRNA, small interfering RNA; Slc8, solute carrier family 8.