Aspalathus linearis suppresses cell survival and proliferation of enzalutamide-resistant prostate cancer cells via inhibition of c-Myc and stability of androgen receptor
Fig 7
GRT treatment suppresses the protein expression, stability, and downstream signaling of AR in C4-2 MDV3100r cells.
(A) C4-2 MDV3100r cells being treated with or without 1 nM DHT or increasing concentration of GRT (0, 50, 100 μg/ml) for 48 h was observed by immunofluorescent microscopy. The column from left to right shows DAPI (blue), F-actin (green), and AR (androgen receptor) (red) images. The magnification of the microscope is 100X and the yellow scale bar represented 100 μm. (B) Expression of AR mRNA in C4-2 MDV3100r cells being treated with 0, 50, 100 μg/ml was examined by qRT-PCR. Asterisk * and *** represents statistically significant difference p < 0.05 and p < 0.001, respectively, between the two groups of cells being compared. (C) C4-2 MDV3100r cells were treated with 10 μg/ml cycloheximide with or without 100 μg/ml GRT for 0, 4, 8, 24, 48 h. Expression of AR protein was examined by Western blotting and the β-actin was used as loading control. (D) LNCaP C4-2 MDV3100r cells were treated with or without 1 nM DHT along with increasing concentration of GRT (0, 50, 100 μg/ml) for 96 h. Abundance of AR and PSA protein was determined by Western blotting. The β-actin was used as loading control.