Molecular Basis of Gene-Gene Interaction: Cyclic Cross-Regulation of Gene Expression and Post-GWAS Gene-Gene Interaction Involved in Atrial Fibrillation
Fig 7
PITX2c and ZFHX3 negatively regulate expression of the CAV1 gene.
HCT116 cells were transfected with siRNA specific for PITX2c or siRNA specific for ZFHX3 and used for isolation of total RNA samples and real-time RT-PCR analysis. A. Real-time RT-PCR analysis for CAV1. Transfection of siRNA for PITX2c or siRNA for ZFHX3 successfully increased expression of CAV1. B, C. Western blot analysis revealed that PITX2c and ZFHX3 increased the expression of the CAV1 protein by 1.79-fold and 1.84-fold, respectively (P = 2.21×10-5, 2.00×10-7). **P<0.01; *<0.05.