Potent and Broad Inhibition of HIV-1 by a Peptide from the gp41 Heptad Repeat-2 Domain Conjugated to the CXCR4 Amino Terminus
Fig 7
CD4 T cells expressing C34-CXCR4 are protected in HIV-1-infected NSG mice.
(Panels A-D). NSG mice were infused with human CD4 T cells that were either untransduced or transduced with C34-CXCR4 or GFP. (Panel A) Engraftment was assessed after 30 days, which was 7 days prior to inoculation with a mix of HIV-1 isolates US1 (R5-tropic) and CMU-02 (X4-tropic). (Panels B and C) CD4 T cell numbers and plasma viral loads are shown 8 days after inoculation. (Panel D) CD4 T cells in spleen are shown at necropsy (Day 28). (Panels E-F) In a separate experiment, NSG mice were infused with CD4 T cells transduced with C34-CXCR4, C34-CCR5, or GFP and following engraftment inoculated with the same HIV-1 viral mix. (Panel E) Numbers of CD4 T cells are shown starting at Day 0, (2 days before inoculation), and at the indicated days after inoculation. (Panels F and G) CD4 T cells in blood and in spleen are shown on the day of necropsy (Day 21).