Dexamethasone treatment of murine auditory hair cells and cochlear explants attenuates tumor necrosis factor-α-initiated apoptotic damage
Fig 7
Attenuation of TNF-α-induced apoptosis through pretreatment of DEX in cochlear explants.
(A) The representative confocal images showing IHCs and OHCs stained with primary antibody to myosin 7a and annexin V in middle-turn cochlear explants (a; control, b; 10 nM DEX, c; 20 ng/ml TNF-α, d; 10 nM DEX pretreatment then 20 ng/ml TNF-α). The Myosin 7a (red) and annexin V (green) were used for counting the number of hair and apoptotic cells, respectively. Scale bars = 20 mm, Original magnification = 400 ×. (B) The graph represents the number of annexin V/myosin 7a double-positive HCs per 160 mm in the middle-turn cochlear explants. Data expressed as mean ± SE of the number of HCs (n = 4 different explants per group); ## P < 0.01, *** P < 0.001, ***; compared with untreated control, ##; TNF-α only versus DEX plus TNF-α.