Streptococcus pneumoniae Cell-Wall-Localized Phosphoenolpyruvate Protein Phosphotransferase Can Function as an Adhesin: Identification of Its Host Target Molecules and Evaluation of Its Potential as a Vaccine
Fig 7
Inhibition of S. pneumoniae adhesion to A549 cells by Eps 1-derived peptide.
S. pneumoniae cells were treated for 1 h with Eps 1-derived peptide and added to A549 cells for 1 h; non-adherent bacteria were removed; and cells were detached with trypsin and plated onto blood agar plates for counting. A) Strain WU2 (p < 0.0001; r = −0.943); B) Strain 3.8DW (p < 0.0001; r = −0.056); C) Strain D39 (p < 0.0001; r = −0.943); D) Strain R6 (p <0.0001; r = −0.406; *t-test p < 0.05). Experiments were performed in 3–6 replicates and repeated at least 3 times. Values are means±SD. *Student's t-test p < 0.05.