Thyrotropin-Releasing Hormone (TRH) Promotes Wound Re-Epithelialisation in Frog and Human Skin
Figure 7
TRH stimulates re-epithelialisation and enhances proliferation in wounded human skin.
(A) Representative H&E stained sections of human skin punch-wounds following 6 days culture with either vehicle control or 10 ng/mL TRH. New epithelial tongues indicated by the white-hatched lines. Scale bars represent 50 µm. (B) Representative images of Ki-67-TUNEL double-stained sections of human skin punch wounds following 6 days culture with either vehicle control or 10 ng/mL TRH. The white-hatched line demarcates new epithelial tongue. (C) The graph displays length measurements of the new epithelial tongue (as demarcated by the white-hatched lines in A) as analysed from H&E stained human skin sections following treatment with vehicle control, 5 ng/mL and 10 ng/mL TRH. (D) Percentage of proliferative (Ki67-positive) cells in the new epithelial tongue of vehicle control, 5 ng/mL and 10 ng/mL TRH-treated human skin wounds. (E) Percentage of apoptotic (TUNEL-positive) cells in the new epithelial tongue of vehicle control, 5 ng/mL and 10 ng/mL TRH-treated human skin wounds. Data are mean ± SEM of 4 female donors. Significance relative to control data at the same time-point denoted by *P<0.05, **P<0.01, ***P<0.001.