Identification and Characterization of a Novel Porin Family Highlights a Major Difference in the Outer Membrane of Chlamydial Symbionts and Pathogens
Figure 7
Purification of PomS from P. amoebophila EBs.
A gel stained with colloidal coomassie is shown; 1, outer membrane fraction after incubation of EBs with n-octyl-POE; 2, outer membrane fraction after precipitation with acetone; 3, column flow through; 4–7, fractions after elution with 0.1, 0.25, 0.3, 0.35 and 0.4 M NaCl. Molecular mass of marker bands (M) in kDa; the expected size of PomS (33.9 kDa) is indicated by an arrow head.