Aspalathus linearis suppresses cell survival and proliferation of enzalutamide-resistant prostate cancer cells via inhibition of c-Myc and stability of androgen receptor
Fig 6
Knockdown of c-Myc increased sensitivity of PC-3 cells to GRT treatment.
(A) Knockdown of c-Myc in PC-3 cells was confirmed by Western blotting. (B) PC-3 cells with control scramble or c-Myc knockdown were treated with increasing concentration of GRT (0, 10, 25, 50, 75, 100 μg/ml) and cell proliferation was examined by proliferation assay. Asterisk * and *** represents statistically significant difference p < 0.05 and p < 0.001, respectively, between the group as compared to control group or the denoted two groups being compared. (C) The protein expression of c-Myc in C4-2 MDV3100r or PC-3 cells being treated with or without 100 μg/ml was examined by Western blotting. The β-Actin was used as loading control.