Generation of an artificial human B cell line test system using Transpo-mAbTM technology to evaluate the therapeutic efficacy of novel antigen-specific fusion proteins
Fig 6
XTT cell viability assay to determine the cytotoxicity of TTC-based proteins.
TTC-reactive REH cells (black) as well as the mock-transfected control REH cells (gray) were used to demonstrate the cytotoxicity of TTC-ETA' (A, ■), GrB(R201K)-TTC (B, ▼) and TTC-MAPTau (C, ●). The cells were incubated with an increasing concentration of the recombinant fusion proteins for 72 h at 37°C and 5% CO2 followed by an XTT cell viability assay. The EC50 value relative to untreated control cells (100% cell viability) and the positive control zeocin (0% cell viability) was calculated using the three-parameter dose-response curve fit equation with GraphPad Prism v5 software. The data are means ± SD of three independent experiments performed in triplicate (n = 3). Statistical significance was calculated by two-way ANOVA followed by Bonferroni’s post-hoc test (*** p < 0.001, ** p < 0.01, * p < 0.05, n.s.–not significant).