APRIL Induces Tumorigenesis and Metastasis of Colorectal Cancer Cells via Activation of the PI3K/Akt Pathway
Figure 6
shAPRIL (sh637) inhibited the invasiveness of SW480 by decreasing the expression and activity of MMPs.
(A) shNTC or shAPRIL cells were treated with 10 µM GM6001 or DMSO (control), and analyzed for their invasion ability with Matrigel coated transwell chambers. The invasion assay was performed three times independently. (B) At 48 h post-transfection, secreted MMP-2 and MMP-9 in the culture supernatants were determined by ELISA. (C) At 48 h post-transfection, cells were harvested and MMP-2, MMP-9 and TIMP-1 mRNA levels were determined by semiquantitative RT-PCR 48 h post-transfection. GAPDH was an internal control. Bars represent means ± SEM from three independent experiments; *P<0.05 compared with shNTC.