The Timing of Stimulation and IL-2 Signaling Regulate Secondary CD8 T Cell Responses
Fig 6
The availability of IL-2 signals impacts the differentiation of 2° CD8 T cells.
‘Early’ and ‘late’ groups of mice were treated with control IgG or high-affinity IL-2 receptor stimulating complex (1.5 μg/mL IL-2: 50μg/mL S4B6) from days 4–6 post-transfer. A) Percentage of 2° effector P14 CD8 T cells in the spleens of mice from ‘early’ and ‘late’ groups. B) Total numbers of 2° effector P14 Thy1.1 CD8 T cells from the spleens of individual mice in the ‘late’ group at day 7 post transfer. Data are presented as mean+ SEM of 3–4 mice per group. C) Representative histograms showing the expression of the CD25 molecule on 2° effector P14 CD8 T cells from the ‘late’ group. Shaded graph represents isotype control staining and open graphs represent specific Ab staining on gated 2° effector Thy1.1 P14 CD8 T cells. D) Representative dot plots showing the expression of KLRG1 and CD127 molecules on 2° effector P14 CD8 T cells at day 7 after transfer. The percentage of 2° effector P14 CD8 T cells expressing a E) KLRG1hi CD127lo or F) KLRG1lo CD127hi phenotype in the spleen of individual mice in the ‘late’ group is shown. Data are presented as mean+ SEM of 3–4 mice per group. G-J) ‘Early’ and ‘late’ groups of mice were treated with control IgG or IL-2 blockade (JES6-1A12, 500μg/mouse) from day 2–5 post-transfer. G) The total number of P14 CD8 T cells from the spleen of individual mice at day 7 after transfer. H) The percentage of BrdU+ P14 CD8 T cells in the spleen. I) The total number of P14 CD8 T cells from the spleens of individual mice at day 7 after transfer. J) The percentage of BrdU+ P14 CD8 T cells in the spleen. Data in G-J) are presented as mean + SEM of 4–5 mice per group. All experiments are representative of 2 independent experiments. The p values are indicated.