Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage
Fig 6
Differential HDX of IL-23 in complex with 2 peptides versus the apo state illustrated on a model of IL-23 built from 5MZV.
Differential HDX of IL-23 in complex with A) 23–446 and B) 23–437. The color (generated using the pymol spectrum command) of each alpha carbon represents the magnitude of the change in calculated H-D exchange rate constant upon binding of the corresponding ligand with purple < = -2.0, blue, green, white = 0.0, yellow, orange, and red > = +2.0 log units. The size of each sphere indicates confidence that the apparent signal is truly non-zero. It is determined based upon the mean and standard deviation of the acceptable models from the Bayesian analysis with 0.25 Å radius indicating zero confidence and 2.0 Å indicating a confidence of 1.0 [22]. The blue and green toward the right of the protein upon binding of 23–446 peptide shows protection to exchange suggestive of binding in that region. Blue and green closer to the center of the structure in the 23–437 peptide suggests binding in that zone.