Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations
Fig 6
Artificial TALEs do not always shift the natural Transcriptional Start Site (TSS) at the OsSWEET14 promoter in N. benthamiana.
Overview of analyzed artificial and natural TALEs that bind to different positions in the OsSWEET14 promoter. The natural OsSWEET14 TSS in rice is marked with a red "A", and the AvrXa7-induced TSS is marked with a grey "G" [20]. Agrobacterium strains delivering the 35S-controlled TALE constructs were co-inoculated into N. benthamiana leaves along with an Agrobacterium strain delivering the reporter construct. 1 dpi leaf discs were harvested and total RNA was extracted. cDNA was produced and used for 5' RACE. The first nucleotide of each identified TSS is labeled with a capital letter. The distance of the TSS to the 3´end of forward-orientated TALE target sequences and to the 5´end of reverse-orientated TALE target sequences as well as the number of analyzed clones ("x") is indicated to the right. TSSs that overlap with the natural OsSWEET14 TSS in rice are marked in red.