Inhibition of Nickel Nanoparticles-Induced Toxicity by Epigallocatechin-3-Gallate in JB6 Cells May Be through Down-Regulation of the MAPK Signaling Pathways
Fig 6
Oxidative stress staining after JB6 cells were treated with Ni NPs alone or Ni NPs + EGCG.
Notes: H2DCFDA (green) and DHE (red) are used for staining general ROS and •O2- produced in the intact cells, respectively. Hoechst 33258 (blue) is a nucleic acid stain. 500 μM H2O2 was used as a positive control for ROS generation. Abbreviations: Ni NPs, nickel nanoparticles; EGCG, epigallocatechin-3-gallate; H2DCFDA, 2',7'-dichlorodihydrofluorescein diacetate; DHE, dihydroethidium; ROS, reactive oxygen species.