Development of a Nuclear Transformation System for Oleaginous Green Alga Lobosphaera (Parietochloris) incisa and Genetic Complementation of a Mutant Strain, Deficient in Arachidonic Acid Biosynthesis
Figure 6
Analysis of L. incisa P127 clones transformed with a pD5Dselect construct.
(A) GC-FID analysis of the fatty acid profiles of P127 mutant, P127-transformant and wild type cells, (B) qRT-PCR analysis of expression of DES5 gene in P127 mutant, P127-transformant and wild type cells. (C) PCR analysis of gDNA isolated from P127 mutant, P127-transformant and wild type cells with primers LiDes5 F3 and BleR, demonstrating transgene integration in conjunction with the ble gene.