The Attenuated Brucella abortus Strain 19 Invades, Persists in, and Activates Human Dendritic Cells, and Induces the Secretion of IL-12p70 but Not IL-23
Figure 6
Failure of supernatants from B. abortus S19-infected DCs to transfer the DC-activating potential to immature, uninfected DCs.
Immature cells were infected with B. abortus S19 (MOI, 20) or left uninfected and cultured for 48 h. Supernatants were harvested and transferred (50% vol/vol) to uninfected, immature DCs, and the phenotype of the cells was determined after 48 h. The expression of typical DC-maturation markers vs. size (FSC) is shown. One representative experiment of three independent experiments with comparable results is shown.