Molecular Basis of Gene-Gene Interaction: Cyclic Cross-Regulation of Gene Expression and Post-GWAS Gene-Gene Interaction Involved in Atrial Fibrillation
Fig 6
PITX2c and ZFHX3 regulate expression of NKX2.5, TBX5, KCNQ1 and SCN1B.
HCT116 cells were transfected with siRNA specific for PITX2c or ZFHX3 and used for isolation of total RNA samples and real-time RT-PCR analysis. Transfection of siRNA for PITX2c increased expression of NKX2.5, TBX5, KCNQ1 and SCN1B. Transfection of siRNA for ZFHX3 increased expression of NKX2.5 and TBX5,but decreased expression of SCN1B. ZFHX3 did not affect on the expression of KCNQ1. Transfection of siRNAs for both PITX2c and ZFHX3 increased expression of NKX2.5 and TBX5.