Laser Microdissection of Narrow Sheath Mutant Maize Uncovers Novel Gene Expression in the Shoot Apical Meristem
Figure 6
In Situ Hybridization Reveals Domain-Specific Expression of Differentially Expressed Maize Genes in Nonmutant and ns1-R Mutant Shoot Apices, Part II
Drawings of SAM expression patterns are modeled in (C and D), (G and H), (K and L), and (N and P). Differentially expressed maize genes are shown in nonmutant (wild type: [A], [E], [I], [M], and [O]) and ns1-R mutant (ns: [B], [F], and [J]) shoot apices. No expression in leaf primordia is portrayed in drawings, since transcripts accumulating in leaves were not LM sampled or reflected in microarray data. Probes were made from maize ESTs: DN232668 (A and B), DY400928 (E and F), AI820200 (I and J), CB381550 (M), and CD650947 (O). Predicted functions are abbreviated as: ara GTPase, GTP-BINDING PROTEIN ARA-3; sugar trans, SUGAR TRANSPORTER PROTEIN; amine oxidase, AMINE OXIDASE1; FtsZ, CELL DIVISION FTSZ PROTEIN; and Yabby, YABBY-RELATED PROTEIN. Numbers denote leaf primordia. Analyses of expression patterns are provided in the text.