Type-I interferons promote innate immune tolerance in macrophages exposed to Mycobacterium ulcerans vesicles
Fig 5
The induction of innate immune tolerance in the presence of mycolactone in FVB/n macrophages is associated with histone methylases.
Macrophages from FVB/N and BALB/c mice were seeded into plates and incubated or not for six hours with vesicles from a mycolactone deficient strain (MEVs NPM) (MOI: 20 000) in the presence of purified mycolactone (Myco) (6 ng/mL). mRNAs were collected and gene expression was measured by RNAseq. (A) A heatmap of genes related to the lysine degradation pathway including most of the enzymes involved in the methylation of histone’s lysine residues. (B) Macrophages from BALB/c mice were seeded into plates and incubated or not for 6 or 24 hours with vesicles from mycolactone deficient strain (MEVs NPM) (MOI: 20 000) +/- purified mycolactone (Myco) (6 ng/mL) and recombinant IFN-β (1 uM). mRNAs were collected and gene expression was measured by qRT-PCR. The expression of setdb2 is shown as fold change over the control condition (medium only). (A) Data are based on three independent experiments. (B) Data represent the mean ± SEM of two independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey post-test (*P < 0.05, ***P >0.001).