Spike substitution T813S increases Sarbecovirus fusogenicity by enhancing the usage of TMPRSS2
Fig 5
The S813T mutation has no effect on S protein interactions with ACE2.
(A) SDS-polyacrylamide gel electrophoresis (PAGE) of SARS2-6P, SARS-2P and Residue 813 substitution S variants. Molecular weight standards are indicated at the left in KD. (B) Competitive ELSIA to detect the binding affinity between S proteins and ACE2. Red line indicated SARS-CoV-2, and blue line indicated SARS-CoV. (C) Neutralization curves for RBD representative antibodies, m396 and X65, with the VSVpp containing parent and mutant S proteins. Each point represents the mean and standard error of 2 independent measurements.